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Tammur J, Sibul H, Ustav E, et al. A bovine papillomavirus-1 based vector restores the function of the low-density lipoprotein receptor in the receptor-deficient CHO-ldlA7 cell line. BMC Mol Biol. 2002;3:5doi: 10.1186/1471-2199-3-5.
Tammur, J., Sibul, H., Ustav, E., Ustav, M., & Metspalu, A. (2002). A bovine papillomavirus-1 based vector restores the function of the low-density lipoprotein receptor in the receptor-deficient CHO-ldlA7 cell line. BMC molecular biology, 35. https://doi.org/10.1186/1471-2199-3-5
Tammur, Jaana, et al. "A bovine papillomavirus-1 based vector restores the function of the low-density lipoprotein receptor in the receptor-deficient CHO-ldlA7 cell line." BMC molecular biology vol. 3 (2002): 5. doi: https://doi.org/10.1186/1471-2199-3-5
Tammur J, Sibul H, Ustav E, Ustav M, Metspalu A. A bovine papillomavirus-1 based vector restores the function of the low-density lipoprotein receptor in the receptor-deficient CHO-ldlA7 cell line. BMC Mol Biol. 2002 Apr 19;3:5. doi: 10.1186/1471-2199-3-5. Epub 2002 Apr 19. PMID: 11967145; PMCID: PMC111063.
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BMC Mol Biol. 2002 Apr 19;3:5. doi: 10.1186/1471-2199-3-5. Epub 2002 Apr 19.

A bovine papillomavirus-1 based vector restores the function of the low-density lipoprotein receptor in the receptor-deficient CHO-ldlA7 cell line.

BMC molecular biology

Jaana Tammur, Hiljar Sibul, Ene Ustav, Mart Ustav, Andres Metspalu

Affiliations

  1. Institute of Molecular and Cell Biology, Tartu University, Estonian Biocentre, 23 Riia St, 51010 Tartu, Estonia. [email protected]

PMID: 11967145 PMCID: PMC111063 DOI: 10.1186/1471-2199-3-5

Abstract

BACKGROUND: The rationale of using bovine papillomavirus-1 (BPV-1) derived vectors in gene therapy protocols lies in their episomal maintenance at intermediate to high copy number, and stable, high-level expression of the gene products. We constructed the BPV-1 based vector harbouring the human low-density lipoprotein receptor (LDLR) gene cDNA and tested its ability to restore the function of the LDLR in the receptor-deficient cell line CHO-ldlA7.

RESULTS: The introduced vector p3.7LDL produced functionally active LDL receptors in the receptor-deficient cell line CHO-ldlA7 during the 32-week period of observation as determined by the internalisation assay with the labelled LDL particles.

CONCLUSION: Bovine papillomavirus type-1 (BPV-1)-derived vectors could be suitable for gene therapy due to their episomal maintenance at intermediate to high copy number and stable, high-level expression of the gene products. The constructed BPV-1 based vector p3.7LDL produced functionally active LDL receptors in the LDLR-deficient cell line CHO-ldlA7 during the 32-week period of observation. In vivo experiments should reveal, whether 1-5% transfection efficiency obtained in the current work is sufficient to bring about detectable and clinically significant lowering of the amount of circulating LDL cholesterol particles.

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