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Schmid KW, Bankfalvi A, Mucke S, et al. Possible Relation of p53 and mdm-2 Oncoprotein Expression in Thyroid Carcinoma: A Molecular-Pathological and Immunohistochemical Study on Paraffin-Embedded Tissue. Endocr Pathol. 1996;7(2):121-130doi: 10.1007/BF02739971.
Schmid, K. W., Bankfalvi, A., Mucke, S., Ofner, D., Riehemann, K., Schroder, S., Stucker, A., Totsch, M., & Dockhorn-Dworniczak, B. (1996). Possible Relation of p53 and mdm-2 Oncoprotein Expression in Thyroid Carcinoma: A Molecular-Pathological and Immunohistochemical Study on Paraffin-Embedded Tissue. Endocrine pathology, 7(2), 121-130. https://doi.org/10.1007/BF02739971
Schmid, Kurt W., et al. "Possible Relation of p53 and mdm-2 Oncoprotein Expression in Thyroid Carcinoma: A Molecular-Pathological and Immunohistochemical Study on Paraffin-Embedded Tissue." Endocrine pathology vol. 7,2 (1996): 121-130. doi: https://doi.org/10.1007/BF02739971
Schmid KW, Bankfalvi A, Mucke S, Ofner D, Riehemann K, Schroder S, Stucker A, Totsch M, Dockhorn-Dworniczak B. Possible Relation of p53 and mdm-2 Oncoprotein Expression in Thyroid Carcinoma: A Molecular-Pathological and Immunohistochemical Study on Paraffin-Embedded Tissue. Endocr Pathol. 1996;7(2):121-130. doi: 10.1007/BF02739971. PMID: 12114639.
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Endocr Pathol. 1996;7(2):121-130. doi: 10.1007/BF02739971.

Possible Relation of p53 and mdm-2 Oncoprotein Expression in Thyroid Carcinoma: A Molecular-Pathological and Immunohistochemical Study on Paraffin-Embedded Tissue.

Endocrine pathology

Kurt W. Schmid, Agnes Bankfalvi, Swantja Mucke, Dietmar Ofner, Kristina Riehemann, Soren Schroder, Andrea Stucker, Martin Totsch, Barbara Dockhorn-Dworniczak

PMID: 12114639 DOI: 10.1007/BF02739971

Abstract

Routinely processed tissues from a series of benign and malignant thyroid lesions were immunohistochemically investigated with antibodies against p53 and mdm-2. p53 was immunolocalized in <10% of nuclei in 2/80 nodular goiters, 2/60 follicular adenomas, 26/68 follicular carcinomas, 7/40 papillary carcinomas, 3/10 "insular" carcinomas, and 10/31 anaplastic carcinomas. More than 10% positively stained nuclei were found in 2 widely invasive follicular, 2 insular, and 15 anaplastic carcinomas. All p53-positive cases showed a concomitant immunohistochemical mdm-2 expression; an immunohistochemical colocalization on serial section was demonstrated in 12 anaplastic carcinomas. Screening by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis of these 12 cases revealed no relevant mutations in the coding regions of exons 2-11 of the p53 gene. Additionally, 1 follicular adenoma, 6 follicular carcinomas (4 minimally and 2 widely invasive), 1 papillary, and 2 poorly differentiated insular carcinomas were mdm-2 positive without immunohistochemically detectable p53 expression. These results provide evidence that wild-type p53 expression in thyroid carcinomas may be associated with mdm-2 induced formation of stable complexes. However, the role of p53 mutations and p53 protein inactivation owing to other factors (e.g., mdm-2) in the progression of thyroid carcinomas is still poorly understood.

References

  1. Histopathology. 1994 Mar;24(3):205-10 - PubMed
  2. Clin Mol Pathol. 1995 Feb;48(1):M12-6 - PubMed
  3. J Pathol. 1994 Dec;174(4):301-7 - PubMed
  4. Cell. 1992 Jun 26;69(7):1237-45 - PubMed
  5. Pathol Res Pract. 1995 May;191(4):304-17 - PubMed
  6. Cancer Res. 1991 Aug 1;51(15):4090-6 - PubMed
  7. Int J Cancer. 1994 Feb 1;56(3):347-53 - PubMed
  8. J Pathol. 1992 Apr;166(4):329-30 - PubMed
  9. Science. 1990 Apr 6;248(4951):76-9 - PubMed
  10. J Pathol. 1994 Nov;174(3):223-8 - PubMed
  11. Jpn J Cancer Res. 1992 Dec;83(12):1293-8 - PubMed
  12. Biotechniques. 1989 Jul-Aug;7(7):736-44 - PubMed
  13. J Clin Invest. 1993 Jan;91(1):179-84 - PubMed
  14. Nature. 1979 Mar 15;278(5701):261-3 - PubMed
  15. Oncogene. 1991 Sep;6(9):1693-7 - PubMed
  16. Jpn J Cancer Res. 1992 Nov;83(11):1113-6 - PubMed
  17. Cell. 1982 Feb;28(2):387-94 - PubMed
  18. Am J Surg Pathol. 1993 Apr;17(4):375-81 - PubMed
  19. J Natl Cancer Inst. 1993 Jun 16;85(12):965-70 - PubMed
  20. Am J Pathol. 1993 Nov;143(5):1389-97 - PubMed
  21. Cancer Res. 1992 Sep 15;52(18):5061-4 - PubMed
  22. Br J Cancer. 1994 Mar;69(3):409-16 - PubMed
  23. Diagn Mol Pathol. 1994 Mar;3(1):9-14 - PubMed
  24. Cancer Res. 1992 Mar 1;52(5):1369-71 - PubMed
  25. Genes Dev. 1990 Jan;4(1):1-8 - PubMed
  26. Genes Dev. 1993 Jul;7(7A):1126-32 - PubMed
  27. Cancer Res. 1990 Dec 1;50(23):7717-22 - PubMed
  28. Semin Cancer Biol. 1993 Jun;4(3):143-52 - PubMed
  29. J Clin Pathol. 1994 Apr;47(4):337-9 - PubMed
  30. J Clin Invest. 1993 Apr;91(4):1753-60 - PubMed

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