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Kleer CG, Bryant BR, Giordano TJ, et al. Genetic Changes in Chromosomes 1p and 17p in Thyroid Cancer Progression. Endocr Pathol. 2000;11(2):137-143doi: 10.1385/ep:11:2:137.
Kleer, C. G., Bryant, B. R., Giordano, T. J., Sobel, M., & Merino, M. J. (2000). Genetic Changes in Chromosomes 1p and 17p in Thyroid Cancer Progression. Endocrine pathology, 11(2), 137-143. https://doi.org/10.1385/ep:11:2:137
Kleer, Celina G., et al. "Genetic Changes in Chromosomes 1p and 17p in Thyroid Cancer Progression." Endocrine pathology vol. 11,2 (2000): 137-143. doi: https://doi.org/10.1385/ep:11:2:137
Kleer CG, Bryant BR, Giordano TJ, Sobel M, Merino MJ. Genetic Changes in Chromosomes 1p and 17p in Thyroid Cancer Progression. Endocr Pathol. 2000;11(2):137-143. doi: 10.1385/ep:11:2:137. PMID: 12114819.
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Endocr Pathol. 2000;11(2):137-143. doi: 10.1385/ep:11:2:137.

Genetic Changes in Chromosomes 1p and 17p in Thyroid Cancer Progression.

Endocrine pathology

Celina G. Kleer, Bonita R. Bryant, Thomas J. Giordano, Mark Sobel, Maria J. Merino

Affiliations

  1. MT (ASCP).

PMID: 12114819 DOI: 10.1385/ep:11:2:137

Abstract

Little is known about the genetic alterations that occur during the progression of thyroid neoplasms. To understand better the biology of thyroid tumors, we investigated several genetic loci in benign and malignant thyroid neoplasms. Forty-one thyroid tumors (6 adenomas, 16 papillary, 14 follicular, and 5 anaplastic carcinomas) were studied. Normal and tumor cells were microdissected from paraffin-embedded tissues. DNA was used for polymerase chain reaction-based loss of heterozygosity (LOH) analysis with the following markers: D1S243 (1p35-36), D1S165 (1p36) and D1S162 (1p32), TP53 (17p13), and INT-2 (11q13). Immunohistochemistry for Ki-67 was performed. The Ki-67 labeling index (LI) was the percentage of positive tumor cells. LOH at 1p was seen in 2 of 5 (40%) informative cases of anaplastic carcinoma (2 of 2 at D1S162 and 1 of 2 at D1S165) and in 2 of 11 (18%) informative cases of follicular carcinoma (2 of 7 at D1S243, 2 of 7 at D1S1654, and 1 of 6 at D1S162). One anaplastic (20%) and two follicular carcinomas (14%) had LOH in at least two of the 1p loci analyzed. None of the adenomas and papillary carcinomas had LOH at these loci. LOH at 17p and 11q13 were infrequent. Ki-67 LI was 1.4, 7, 16, and 65% in adenomas, papillary, follicular, and anaplastic carcinomas, respectively. Allelic loss at 1p may occur in aggressive types of thyroid carcinoma and may be a marker of poor prognosis. LOH at 1p may represent a late genetic event in thyroid carcinogenesis. LOH at 17p and 11q13 (MEN gene locus) is uncommon in thyroid neoplasms.

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