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Cheng XD, Xie HY, Qian RL. Effect of IL-3 on Globin Gene Expression in a Human Erythroleukemia Cell Line. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(4):385-389
Cheng, X. D., Xie, H. Y., & Qian, R. L. (1999). Effect of IL-3 on Globin Gene Expression in a Human Erythroleukemia Cell Line. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 31(4), 385-389.
Cheng, Xiao-Dong, et al. "Effect of IL-3 on Globin Gene Expression in a Human Erythroleukemia Cell Line." Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica vol. 31,4 (1999): 385-389.
Cheng XD, Xie HY, Qian RL. Effect of IL-3 on Globin Gene Expression in a Human Erythroleukemia Cell Line. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(4):385-389. PMID: 12114990.
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Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(4):385-389.

Effect of IL-3 on Globin Gene Expression in a Human Erythroleukemia Cell Line.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Xiao-Dong Cheng, Heng-Yue Xie, Ruo-Lan Qian

Affiliations

  1. Shanghai Institute of Cell Biology, the Chinese Academy of Sciences, Shanghai 200031, China. [email protected]

PMID: 12114990

Abstract

A human erythroleukemia cell line(K562 cells)was used as a model to study the effect of interleukin-3(IL-3) on human globin gene expression in the cells. The results showed that the beta-globin gene was not expressed in uninduced K562 cells however, it was expressed when K562 cells were induced for 3 or 5 days by IL-3. The expression of alpha- and gamma-globin gene were not much different between IL-3 induced and uninduced K562 cells. Using the method of benzidine-dyeing, it was observed that the percentage of blue cells was significantly increased when K562 cells were induced by IL-3. It suggested that IL-3 could not only activate the exp-ression of beta-globin gene in K562 cells, but also induce the synthesis of hemoglobin. Therefore, IL-3 may play a critical role in inducing K562 cells to terminal differentiation.

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