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Wallstedt A, Sommarin M, Nilsson MC, et al. The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. Physiol Plant. 2001;113(3):368-376doi: 10.1034/j.1399-3054.2001.1130310.x.
Wallstedt, A., Sommarin, M., Nilsson, M. C., Munson, A. D., & Margolis, H. A. (2001). The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. Physiologia plantarum, 113(3), 368-376. https://doi.org/10.1034/j.1399-3054.2001.1130310.x
Wallstedt, Anna, et al. "The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III." Physiologia plantarum vol. 113,3 (2001): 368-376. doi: https://doi.org/10.1034/j.1399-3054.2001.1130310.x
Wallstedt A, Sommarin M, Nilsson MC, Munson AD, Margolis HA. The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. Physiol Plant. 2001 Nov;113(3):368-376. doi: 10.1034/j.1399-3054.2001.1130310.x. PMID: 12060282.
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Physiol Plant. 2001 Nov;113(3):368-376. doi: 10.1034/j.1399-3054.2001.1130310.x.

The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III.

Physiologia plantarum

Anna Wallstedt, Marianne Sommarin, Marie-Charlotte Nilsson, Alison D. Munson, Hank A. Margolis

Affiliations

  1. Department of Ecology, Lund University, Lund, SE-223 62 Lund, Sweden Département des sciences du bois et de la forêt, Université Laval, Québec, G1K 7P4, Canada Department of Plant Biochemistry, Lund University, SE-221 00 Lund, Sweden Department of Forest Vegetation Ecology, Swedish University of Agricultural Sciences, SE-901 83 Umeå, Sweden.

PMID: 12060282 DOI: 10.1034/j.1399-3054.2001.1130310.x

Abstract

In northern Sweden, plants growing in association with the clonal dwarf shrub Empetrum hermaphroditum usually exhibit limited growth and are N-depleted. Previous studies suggest that this negative effect by E. hermaphroditum may be explained, at least in part, by the release of phenolic compounds, particularly the dihydrostilbene, batatasin-III from foliage to soil. In the present work, we investigated whether batatasin-III has the potential to interfere with NH4+ uptake in birch (Betula pendula) roots. Excised birch roots were exposed to batatasin-III during brief periods in 15NH4+ solutions, and then analyzed for labeled N. Batatasin-III inhibited N-NH4+ uptake by 28, 89 and 95% compared with the control, when roots were treated with 0.1, 1.0 and 2.8 mM of batatasin-III, respectively. The effect of 1.0-mM batatasin-III was greater at pH 4.2 than at pH 6.8. In addition, the inhibition of N-NH4+ uptake by batatasin-III was not reversed after rinsing the roots in water and transferring them to a batatasin-III free solution. Furthermore, birch seedlings immersed in a 1.0-mM batatasin-III solution for 2 h, and then replanted in pots with soil, had decreased growth, such that 10 weeks after treatment, the dry mass of both shoots and roots was reduced by 74 and 73%, respectively, compared with control seedlings. This suggests that a brief exposure to batatasin-III may have a long-term inhibitory effect on whole plant growth. Using plasma membrane vesicles isolated from easily extractable spinach (Spinacia oleracea) leaves, it was found that batatasin-III strongly inhibited proton pumping in isolated plasma membrane vesicles, while it only slightly inhibited ATP hydrolytic activity. The uncoupling of proton pumping from ATP hydrolytic activity suggests that batatasin-III disturbs membrane integrity. This hypothesis was further supported by a greater efflux of ions from birch roots immersed in a batatasin-III solution than from roots in a control solution.

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