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Lebedev N, Van Cleve B, Armstrong G, et al. Chlorophyll Synthesis in a Deetiolated (det340) Mutant of Arabidopsis without NADPH-Protochlorophyllide (PChlide) Oxidoreductase (POR) A and Photoactive PChlide-F655. Plant Cell. 1995;7(12):2081-2090doi: 10.1105/tpc.7.12.2081.
Lebedev, N., Van Cleve, B., Armstrong, G., & Apel, K. (1995). Chlorophyll Synthesis in a Deetiolated (det340) Mutant of Arabidopsis without NADPH-Protochlorophyllide (PChlide) Oxidoreductase (POR) A and Photoactive PChlide-F655. The Plant cell, 7(12), 2081-2090. https://doi.org/10.1105/tpc.7.12.2081
Lebedev, N., et al. "Chlorophyll Synthesis in a Deetiolated (det340) Mutant of Arabidopsis without NADPH-Protochlorophyllide (PChlide) Oxidoreductase (POR) A and Photoactive PChlide-F655." The Plant cell vol. 7,12 (1995): 2081-2090. doi: https://doi.org/10.1105/tpc.7.12.2081
Lebedev N, Van Cleve B, Armstrong G, Apel K. Chlorophyll Synthesis in a Deetiolated (det340) Mutant of Arabidopsis without NADPH-Protochlorophyllide (PChlide) Oxidoreductase (POR) A and Photoactive PChlide-F655. Plant Cell. 1995 Dec;7(12):2081-2090. doi: 10.1105/tpc.7.12.2081. PMID: 12242369; PMCID: PMC161063.
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Plant Cell. 1995 Dec;7(12):2081-2090. doi: 10.1105/tpc.7.12.2081.

Chlorophyll Synthesis in a Deetiolated (det340) Mutant of Arabidopsis without NADPH-Protochlorophyllide (PChlide) Oxidoreductase (POR) A and Photoactive PChlide-F655.

The Plant cell

N. Lebedev, B. Van Cleve, G. Armstrong, K. Apel

Affiliations

  1. Institute for Plant Sciences, Swiss Federal Institute of Technology Zurich (ETH), Universitatstrasse 2, CH-8092 Zurich, Switzerland.

PMID: 12242369 PMCID: PMC161063 DOI: 10.1105/tpc.7.12.2081

Abstract

Chlorophyll (Chl) synthesis in Arabidopsis is controlled by two light-dependent NADPH-protochlorophyllide (PChlide) oxidoreductases (PORs), one (POR A) that is active transiently in etiolated seedlings at the beginning of illumination and another (POR B) that also operates in green plants. The function of these two enzymes during the light-induced greening of dark-grown seedlings has been studied in the wild type and a deetiolated (det340) mutant of Arabidopsis. One of the consequences of the det mutation is that POR A is constitutively down-regulated, and therefore, synthesis of the POR A enzyme is shut off. When grown in the dark, the det340 mutant lacks POR A and the photoactive PChlide-F655 species but maintains the second PChlide reductase, POR B. Previously, photoactive PChlide-F655 has often been considered to be the only PChlide form that leads to Chl formation. Despite its deficiency in POR A and photoactive PChlide-F655, the det340 mutant is able to green when placed in the light. Chl accumulation, however, proceeds abnormally. At the beginning of illumination, seedlings of det340 mutants are extremely susceptible to photooxidative damage and accumulate Chl only at extremely low light intensities. They form core complexes of photosystems I and II but are almost completely devoid of light-harvesting structures. The results of this study demonstrate that in addition to the route of Chl synthesis that has been studied extensively in illuminated dark-grown wild-type plants, a second branch of Chl synthesis exists that is driven by POR B and does not require POR A.

References

  1. Nucleic Acids Res. 1986 May 27;14(10):4051-64 - PubMed
  2. FEBS Lett. 1992 Aug 31;309(1):73-7 - PubMed
  3. Plant Mol Biol. 1992 Mar;18(5):967-72 - PubMed
  4. Planta. 1993;190(4):536-45 - PubMed
  5. Plant Physiol. 1995 Mar;107(3):873-83 - PubMed
  6. Plant Physiol. 1994 Oct;106(2):415-420 - PubMed
  7. Biochem J. 1978 Sep 15;174(3):681-92 - PubMed
  8. Plant Cell. 1994 May;6(5):613-28 - PubMed
  9. Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3254-8 - PubMed
  10. Mol Gen Genet. 1994 Aug 2;244(3):242-52 - PubMed
  11. Eur J Biochem. 1980 Oct;111(1):251-8 - PubMed
  12. Biochim Biophys Acta. 1994 Feb 8;1184(1):1-19 - PubMed
  13. Eur J Biochem. 1981 Nov;120(1):89-93 - PubMed
  14. Planta. 1995;197(3):490-500 - PubMed
  15. Plant Physiol. 1982 Oct;70(4):1019-25 - PubMed
  16. Plant Mol Biol. 1991 Apr;16(4):615-25 - PubMed
  17. Eur J Biochem. 1984 Jan 2;138(1):201-7 - PubMed
  18. Cell. 1989 Sep 8;58(5):991-9 - PubMed
  19. Eur J Biochem. 1981 Nov;120(1):95-103 - PubMed
  20. Plant Physiol. 1995 Aug;108(4):1505-17 - PubMed
  21. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5542-6 - PubMed
  22. Plant Mol Biol. 1989 Nov;13(5):583-93 - PubMed

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