Display options
Cite
Moreno-Herrero F, Valpuesta JM, Pérez M, et al. Characterization by atomic force microscopy and cryoelectron microscopy of tau polymers assembled in Alzheimer's disease. J Alzheimers Dis. 2001;3(5):443-451doi: 10.3233/jad-2001-3502.
Moreno-Herrero, F., Valpuesta, J. M., Pérez, M., Colchero, J., Barö, A. M., Avila, J., & Montejo De Garcini, E. (2001). Characterization by atomic force microscopy and cryoelectron microscopy of tau polymers assembled in Alzheimer's disease. Journal of Alzheimer's disease : JAD, 3(5), 443-451. https://doi.org/10.3233/jad-2001-3502
Moreno-Herrero, Fernando, et al. "Characterization by atomic force microscopy and cryoelectron microscopy of tau polymers assembled in Alzheimer's disease." Journal of Alzheimer's disease : JAD vol. 3,5 (2001): 443-451. doi: https://doi.org/10.3233/jad-2001-3502
Moreno-Herrero F, Valpuesta JM, Pérez M, Colchero J, Barö AM, Avila J, Montejo De Garcini E. Characterization by atomic force microscopy and cryoelectron microscopy of tau polymers assembled in Alzheimer's disease. J Alzheimers Dis. 2001 Oct;3(5):443-451. doi: 10.3233/jad-2001-3502. PMID: 12214029.
Copy Download .nbib Format: NLM
Share it on

J Alzheimers Dis. 2001 Oct;3(5):443-451. doi: 10.3233/jad-2001-3502.

Characterization by atomic force microscopy and cryoelectron microscopy of tau polymers assembled in Alzheimer's disease.

Journal of Alzheimer's disease : JAD

Fernando Moreno-Herrero, José M. Valpuesta, Mar Pérez, Jaime Colchero, Arturo M. Barö, Jesús Avila, Esteban Montejo De Garcini

Affiliations

  1. Departamento de Física de la Materia Condensada, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain.

PMID: 12214029 DOI: 10.3233/jad-2001-3502

Abstract

The structure of the Paired Helical filaments (PHF), a polymer of the microtubule associated protein tau, has been studied by Atomic Force Microscopy (AFM) and by cryoelectron microscopy. Mica and graphite were used as substrates in the AFM analysis with no differences in the results. A banding pattern of 8-12 nm width within the helical structure is found when detailed analysis of the data is performed. High AFM resolution images obtained by using an ultra sharp tip confirm the previous results and suggest that the structures observed are compatible with a helical ribbon made up of two parallel strands. These results were confirmed by cryoelectron microscopy experiments.

Publication Types