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Gray JE, McClure BA, Bonig I, et al. Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes. Plant Cell. 1991;3(3):271-283doi: 10.1105/tpc.3.3.271.
Gray, J. E., McClure, B. A., Bonig, I., Anderson, M. A., & Clarke, A. E. (1991). Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes. The Plant cell, 3(3), 271-283. https://doi.org/10.1105/tpc.3.3.271
Gray, J. E., et al. "Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes." The Plant cell vol. 3,3 (1991): 271-283. doi: https://doi.org/10.1105/tpc.3.3.271
Gray JE, McClure BA, Bonig I, Anderson MA, Clarke AE. Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes. Plant Cell. 1991 Mar;3(3):271-283. doi: 10.1105/tpc.3.3.271. PMID: 12324597; PMCID: PMC159998.
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Plant Cell. 1991 Mar;3(3):271-283. doi: 10.1105/tpc.3.3.271.

Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

The Plant cell

J. E. Gray, B. A. McClure, I. Bonig, M. A. Anderson, A. E. Clarke

Affiliations

  1. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia.

PMID: 12324597 PMCID: PMC159998 DOI: 10.1105/tpc.3.3.271

Abstract

The products of the S-locus expressed in female tissues of Nicotiana alata are ribonucleases (S-RNases). The arrest of growth of incompatible pollen tubes in styles may result from entry of the S-RNase into the pollen tube and degradation of pollen tube RNA. We investigated the action of isolated S-RNases on pollen tubes grown in vitro and found that S-RNase is taken up by the pollen without substantial alteration. The S-RNases inhibit incorporation of exogenously added radioactive amino acids into protein by the germinated pollen. The S-RNases also inhibit in vitro translation of pollen tube RNA in a wheat germ cell-free extract. We found no evidence for a specific mRNA substrate for the S-RNases, which implies that if RNase activity is involved in the control of self-incompatibility, allelic specificity is more likely to depend on the selective uptake of S-RNases into pollen tubes or their selective activation or inactivation by pollen factors, rather than cleavage of a specific substrate. Heat treating S2-RNase largely destroys its RNase activity but increases its inhibitory effect on in vitro pollen tube growth. This effect is not due to an increased uptake of S2-RNase by the pollen but is associated with a greatly enhanced accumulation of S2-RNase on the outer surface of the pollen grains.

References

  1. Plant Cell. 1990 Aug;2(8):815-26 - PubMed
  2. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  3. Nature. 1989 Dec 21-28;342(6252):955-7 - PubMed
  4. J Biochem. 1988 Mar;103(3):408-18 - PubMed
  5. J Ultrastruct Res. 1969 Jan;26(1):31-43 - PubMed
  6. Eur J Biochem. 1988 Oct 1;176(3):683-97 - PubMed
  7. Plant Cell. 1989 May;1(5):501-510 - PubMed
  8. Plant Cell. 1989 May;1(5):493-9 - PubMed
  9. Plant Cell. 1989 May;1(5):483-91 - PubMed
  10. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  11. Science. 1990 Nov 16;250(4983):937-41 - PubMed

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