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Torgersen J, Nourizadeh-Lillabadi R, Husebye H, et al. In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene. BMC Genomics. 2002;3(1):25doi: 10.1186/1471-2164-3-25.
Torgersen, J., Nourizadeh-Lillabadi, R., Husebye, H., & Aleström, P. (2002). In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene. BMC genomics, 3(1), 25. https://doi.org/10.1186/1471-2164-3-25
Torgersen, Jacob, et al. "In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene." BMC genomics vol. 3,1 (2002): 25. doi: https://doi.org/10.1186/1471-2164-3-25
Torgersen J, Nourizadeh-Lillabadi R, Husebye H, Aleström P. In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene. BMC Genomics. 2002 Aug 21;3(1):25. doi: 10.1186/1471-2164-3-25. PMID: 12188930; PMCID: PMC126252.
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BMC Genomics. 2002 Aug 21;3(1):25. doi: 10.1186/1471-2164-3-25.

In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene.

BMC genomics

Jacob Torgersen, Rasoul Nourizadeh-Lillabadi, Harald Husebye, Peter Aleström

Affiliations

  1. Department of Biochemistry, Physiology and Nutrition, Norwegian School of Veterinary Science, PO Box 8146 Dep, N-0033 Oslo, Norway. [email protected]

PMID: 12188930 PMCID: PMC126252 DOI: 10.1186/1471-2164-3-25

Abstract

BACKGROUND: Gonadotropin releasing hormone (GnRH) is responsible for stimulation of gonadotropic hormone (GtH) in the hypothalamus-pituitary-gonadal axis (HPG). The regulatory mechanisms responsible for brain specificity make the promoter attractive for in silico analysis and reporter gene studies in zebrafish (Danio rerio).

RESULTS: We have characterized a zebrafish [Trp7, Leu8] or salmon (s) GnRH variant, gnrh3. The gene includes a 1.6 Kb upstream regulatory region and displays the conserved structure of 4 exons and 3 introns, as seen in other species. An in silico defined enhancer at -976 in the zebrafish promoter, containing adjacent binding sites for Oct-1, CREB and Sp1, was predicted in 2 mammalian and 5 teleost GnRH promoters. Reporter gene studies confirmed the importance of this enhancer for cell specific expression in zebrafish. Interestingly the promoter of human GnRH-I, known as mammalian GnRH (mGnRH), was shown capable of driving cell specific reporter gene expression in transgenic zebrafish.

CONCLUSIONS: The characterized zebrafish Gnrh3 decapeptide exhibits complete homology to the Atlantic salmon (Salmo salar) GnRH-III variant. In silico analysis of mammalian and teleost GnRH promoters revealed a conserved enhancer possessing binding sites for Oct-1, CREB and Sp1. Transgenic and transient reporter gene expression in zebrafish larvae, confirmed the importance of the in silico defined zebrafish enhancer at -976. The capability of the human GnRH-I promoter of directing cell specific reporter gene expression in zebrafish supports orthology between GnRH-I and GnRH-III.

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