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Atkins CA, Smith P, Storer PJ. Reexamination of the Intracellular Localization of de Novo Purine Synthesis in Cowpea Nodules. Plant Physiol. 1997;113(1):127-135doi: 10.1104/pp.113.1.127.
Atkins, C. A., Smith, P., & Storer, P. J. (1997). Reexamination of the Intracellular Localization of de Novo Purine Synthesis in Cowpea Nodules. Plant physiology, 113(1), 127-135. https://doi.org/10.1104/pp.113.1.127
Atkins, C. A., et al. "Reexamination of the Intracellular Localization of de Novo Purine Synthesis in Cowpea Nodules." Plant physiology vol. 113,1 (1997): 127-135. doi: https://doi.org/10.1104/pp.113.1.127
Atkins CA, Smith P, Storer PJ. Reexamination of the Intracellular Localization of de Novo Purine Synthesis in Cowpea Nodules. Plant Physiol. 1997 Jan;113(1):127-135. doi: 10.1104/pp.113.1.127. PMID: 12223595; PMCID: PMC158123.
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Plant Physiol. 1997 Jan;113(1):127-135. doi: 10.1104/pp.113.1.127.

Reexamination of the Intracellular Localization of de Novo Purine Synthesis in Cowpea Nodules.

Plant physiology

C. A. Atkins, PMC. Smith, P. J. Storer

Affiliations

  1. Department of Botany, University of Western Australia, Nedlands, WA 6907 Australia.

PMID: 12223595 PMCID: PMC158123 DOI: 10.1104/pp.113.1.127

Abstract

Sucrose and Percoll density gradient centrifugation were used to separate organelles from the central zone tissue of cowpea (Vigna unguiculata L. Walp. cv Vita 3: Bradyrhizobium strain CB 756) nodules. Enzyme activity analysis has shown that both plastids and mitochondria have a full complement of enzymes for de novo purine synthesis. In vitro activities of individual component enzymes (glycinamide ribonucleotide synthetase, EC 6.3.4.13; glycinamide ribonucleotide transformylase, EC 2.1.2.2; aminoimidazole ribonucleotide synthetase, EC 6.3.3.1; aminoimidazole carboxamide ribonucleotide transformylase, EC 6.3.2.6; and adenylosuccinate-AMP lyase, EC 4.3.2.2) as well as of the whole purine pathway (from ribose-5-phosphate to inosine monophosphate) were similar in the two organelles. No significant cytosolic or bacteroidal activity of any of the purine pathway enzymes was detected on assay. These findings are contrary to earlier studies (M.J. Boland, K.R. Schubert [1983] Arch Biochem Biophys 220: 179-187; B.J. Shelp C.A. Atkins, P.J. Storer, D.T. Canvin [1983] Arch Biochem Biophys 224: 429-441) that concluded that enhanced expression of purine synthesis in nodules of ureide-forming species is localized to plastids. Significantly increased recovery of activity of key pathway enzymes (particularly of labile aminoimidazole ribonucleotide synthetase) coupled with improved assay methods and the use of Percoll in addition to sucrose for gradient centrifugation have together contributed to much higher reaction rates and more definitive analyses of particulate fractions.

References

  1. Biochemistry. 1986 Jul 29;25(15):4356-65 - PubMed
  2. J Biol Chem. 1992 Jul 5;267(19):13528-34 - PubMed
  3. Plant J. 1994 Jul;6(1):113-21 - PubMed
  4. Biochemistry. 1977 Mar 22;16(6):1093-102 - PubMed
  5. Plant Physiol. 1995 Oct;109(2):353-361 - PubMed
  6. Biochemistry. 1981 Jan 20;20(2):337-44 - PubMed
  7. Comput Appl Biosci. 1995 Aug;11(4):441-7 - PubMed
  8. Eur J Biochem. 1989 Apr 1;180(3):535-45 - PubMed
  9. Arch Biochem Biophys. 1983 Jul 15;224(2):429-41 - PubMed
  10. Plant Physiol. 1982 Jul;70(1):55-60 - PubMed
  11. Prog Nucleic Acid Res Mol Biol. 1992;42:259-87 - PubMed
  12. Plant Physiol. 1978 Jul;62(1):134-8 - PubMed
  13. Plant Physiol. 1988 Jan;86(1):204-7 - PubMed
  14. Plant Physiol. 1991 Jul;96(3):728-36 - PubMed
  15. Biochemistry. 1986 Jul 29;25(15):4366-71 - PubMed
  16. J Biol Chem. 1990 Aug 25;265(24):14227-33 - PubMed
  17. J Biol Chem. 1969 Jul 10;244(13):3507-13 - PubMed
  18. Plant Mol Biol. 1994 Oct;26(1):529-33 - PubMed
  19. Plant J. 1995 Jan;7(1):77-86 - PubMed
  20. Plant Physiol. 1986 Jun;81(2):553-7 - PubMed
  21. Biochemistry. 1989 Mar 21;28(6):2459-71 - PubMed

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