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Demidchik V, Sokolik A, Yurin V. The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma. Plant Physiol. 1997;114(4):1313-1325doi: 10.1104/pp.114.4.1313.
Demidchik, V., Sokolik, A., & Yurin, V. (1997). The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma. Plant physiology, 114(4), 1313-1325. https://doi.org/10.1104/pp.114.4.1313
Demidchik, V., et al. "The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma." Plant physiology vol. 114,4 (1997): 1313-1325. doi: https://doi.org/10.1104/pp.114.4.1313
Demidchik V, Sokolik A, Yurin V. The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma. Plant Physiol. 1997 Aug;114(4):1313-1325. doi: 10.1104/pp.114.4.1313. PMID: 12223773; PMCID: PMC158424.
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Plant Physiol. 1997 Aug;114(4):1313-1325. doi: 10.1104/pp.114.4.1313.

The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma.

Plant physiology

V. Demidchik, A. Sokolik, V. Yurin

Affiliations

  1. Department of Physiology and Biochemistry of Plants, Biological Faculty, Belarus State University, Skaryna Avenue 4, 220050, Minsk, Republic of Belarus.

PMID: 12223773 PMCID: PMC158424 DOI: 10.1104/pp.114.4.1313

Abstract

Changes in plasmalemma permeability caused by excessive Cu2+ levels were examined in cells of a freshwater alga (Nitella flexilis) using a conventional microelectrode voltage-clamp technique. A rapid Cu2+-induced increase of plasmalemma conductance starting from 5 [mu]M Cu2+ was shown. Cu2+-induced plasmalemma conductance (ClGm) was nonselective and potential-independent, resembling the conductance of nonselective ionic leakage of the plasmalemma. The K+ channel conductance was shown to be unaltered by Cu2+, and a decrease in plasmalemma Cl- channel conductance at Cu2+ concentrations above 5 [mu]M was found. The depression of Cl- channels and ClGm were time-, dosage-, and Ca2+-dependent processes, revealing a great similarity in all parameters, with Ca2+ causing the preventive effect by shifting the effective Cu2+ concentrations to higher levels. This phenomenon may be explained by the same Cu2+-modified target on the plasmalemma both for ClGm and Cl- channel depression. In addition, a reversible, inhibitory effect of Cu2+ (>10 [mu]M) on the light-stimulated H+-ATPase electrogenic pump in the plasmalemma was demonstrated. This effect was Ca2+- independent, which made it possible to distinguish it from ClGm. Therefore, the Cu2+-induced dramatic alterations in plant cell plasmalemma permeability are caused mainly by nonselective conductance increases and electrogenic pump inhibition.

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