Display options
Cite
Huang FY, Philosoph-Hadas S, Meir S, et al. Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence. Plant Physiol. 1997;115(1):51-60doi: 10.1104/pp.115.1.51.
Huang, F. Y., Philosoph-Hadas, S., Meir, S., Callaham, D. A., Sabato, R., Zelcer, A., & Hepler, P. K. (1997). Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence. Plant physiology, 115(1), 51-60. https://doi.org/10.1104/pp.115.1.51
Huang, F. Y., et al. "Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence." Plant physiology vol. 115,1 (1997): 51-60. doi: https://doi.org/10.1104/pp.115.1.51
Huang FY, Philosoph-Hadas S, Meir S, Callaham DA, Sabato R, Zelcer A, Hepler PK. Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence. Plant Physiol. 1997 Sep;115(1):51-60. doi: 10.1104/pp.115.1.51. PMID: 12223791; PMCID: PMC158459.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1997 Sep;115(1):51-60. doi: 10.1104/pp.115.1.51.

Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence.

Plant physiology

F. Y. Huang, S. Philosoph-Hadas, S. Meir, D. A. Callaham, R. Sabato, A. Zelcer, P. K. Hepler

Affiliations

  1. Department of Biology, University of Massachusetts, Amherst, Massachusetts, 01003 (F.-Y.H., D.A.C., P.K.H.).

PMID: 12223791 PMCID: PMC158459 DOI: 10.1104/pp.115.1.51

Abstract

The ability to maintain the cytoplasmic Ca2+ concentration ([Ca2+]cyt) at homeostatic levels has been examined during leaf senescence in detached parsley (Petroselinum crispum) leaves. Fluorescence ratiometric imaging of mesophyll cells isolated from parsley leaves at various senescence stages and loaded with the Ca2+ indicator fura-2 has revealed a distinct elevation of [Ca2+]cyt, which was positively correlated with the progress of leaf senescence. This initial increase of [Ca2+]cyt, which was first observed in cells isolated from 3-d-senescent leaves, occurred 1 d before or in parallel with changes in two established senescence parameters, chlorophyll loss and lipid peroxidation. However, the [Ca2+]cyt elevation followed by 2 d the initial increase in the senescence-associated proteolysis. Whereas the [Ca2+]cyt of nonsenescent cells remained at the basal level, the elevated [Ca2+]cyt of the senescent cells was a long-lasting effect. Experimental retardation of senescence processes, achieved by pretreatment of detached leaves with the cytokinin benzyladenine, resulted in maintenance of homeostatic levels of [Ca2+]cyt in cells isolated from 3-d-senescent leaves. These observations demonstrate for the first time to our knowledge a correlation between elevated [Ca2+]cyt and the process of senescence in parsley leaves. Such senescence-associated elevation of [Ca2+]cyt, which presumably results from a loss of the cell's capability to extrude Ca2+, may serve as a signal inducing subsequent deteriorative processes.

References

  1. Plant Physiol. 1949 Jan;24(1):1-15 - PubMed
  2. Plant Cell. 1994 Dec;6(12):1815-28 - PubMed
  3. Plant Physiol. 1992 Aug;99(4):1693-4 - PubMed
  4. Plant Physiol. 1973 Sep;52(3):236-9 - PubMed
  5. Plant Physiol. 1982 Jan;69(1):187-91 - PubMed
  6. Plant Physiol. 1984 Jun;75(2):329-35 - PubMed
  7. Plant Physiol. 1990 Jul;93(3):841-5 - PubMed
  8. Trends Biochem Sci. 1989 May;14(5):187-92 - PubMed
  9. CRC Crit Rev Plant Sci. 1987;6(1):47-103 - PubMed
  10. J Biol Chem. 1985 Mar 25;260(6):3440-50 - PubMed
  11. Plant Physiol. 1988 Oct;88(2):295-302 - PubMed
  12. Dev Biol. 1983 Sep;99(1):41-9 - PubMed
  13. Cell Calcium. 1991 Nov;12 (10 ):675-81 - PubMed

Publication Types