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Tam YY, Slovin JP, Cohen JD. Selection and Characterization of [alpha]-Methyltryptophan-Resistant Lines of Lemna gibba Showing a Rapid Rate of Indole-3-Acetic Acid Turnover. Plant Physiol. 1995;107(1):77-85doi: 10.1104/pp.107.1.77.
Tam, Y. Y., Slovin, J. P., & Cohen, J. D. (1995). Selection and Characterization of [alpha]-Methyltryptophan-Resistant Lines of Lemna gibba Showing a Rapid Rate of Indole-3-Acetic Acid Turnover. Plant physiology, 107(1), 77-85. https://doi.org/10.1104/pp.107.1.77
Tam, Y. Y., et al. "Selection and Characterization of [alpha]-Methyltryptophan-Resistant Lines of Lemna gibba Showing a Rapid Rate of Indole-3-Acetic Acid Turnover." Plant physiology vol. 107,1 (1995): 77-85. doi: https://doi.org/10.1104/pp.107.1.77
Tam YY, Slovin JP, Cohen JD. Selection and Characterization of [alpha]-Methyltryptophan-Resistant Lines of Lemna gibba Showing a Rapid Rate of Indole-3-Acetic Acid Turnover. Plant Physiol. 1995 Jan;107(1):77-85. doi: 10.1104/pp.107.1.77. PMID: 12228344; PMCID: PMC161170.
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Plant Physiol. 1995 Jan;107(1):77-85. doi: 10.1104/pp.107.1.77.

Selection and Characterization of [alpha]-Methyltryptophan-Resistant Lines of Lemna gibba Showing a Rapid Rate of Indole-3-Acetic Acid Turnover.

Plant physiology

Y. Y. Tam, J. P. Slovin, J. D. Cohen

Affiliations

  1. Department of Botany, University of Maryland, College Park, Maryland 20742 (Y.Y.T.).

PMID: 12228344 PMCID: PMC161170 DOI: 10.1104/pp.107.1.77

Abstract

Turnover rate is an important aspect of the regulation of plant processes by plant growth substances. To study turnover of indole-3-acetic acid (IAA), two [alpha]-methyltryptophan-resistant lines (MTR1 and MTR2) of Lemna gibba were generated by nitrosomethyl urea treatment of an inbred line derived from L. gibba G-3. In this report we describe: (a) the development of a selection system using this near isogenic line of L. gibba; (b) techniques for chemical mutation of the lines and selection for [alpha]-methyltryptophan resistance; and (c) the partial characterization of the selected lines. MTR lines contained 3-fold higher levels of anthranilate synthase activity. The enzyme in the MTR lines required higher levels of tryptophan for feedback inhibition. MTR lines also contained 8-fold higher levels of tryptophan, 3-fold higher levels of free IAA, and similar levels of total IAA compared to the inbred line. Turnover rates in the inbred and selected lines were calculated, using the first-order rate equation, based on the decrease over time in isotopic enrichment of I3C6-IAA introduced into L. gibba during a 1-h pulse period. Isotope enrichment in IAA was determined by using gas chromatography-mass spectrometry. Both MTR lines had an approximately 10-fold higher rate of IAA turnover than the parent inbred line.

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