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Shen JL, Ye YJ, Gong ZX. Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(5):515-519
Shen, J. L., Ye, Y. J., & Gong, Z. X. (1998). Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 30(5), 515-519.
Shen, Jia-Li, et al. "Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus." Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica vol. 30,5 (1998): 515-519.
Shen JL, Ye YJ, Gong ZX. Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(5):515-519. PMID: 12168024.
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Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(5):515-519.

Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Jia-Li Shen, Yong-Jun Ye, Zu-Xun Gong

Affiliations

  1. Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. [email protected]

PMID: 12168024

Abstract

The cDNA fragment located downstream of the WRSV N protein gene was obtained from the cDNA library of WRSV by hybridization with a 24 nucleotides sequence fragment of the 3' end of mRNA of the N protein. The cDNA fragment was sequenced and on ORF encoding a 40 kD protein was found. The gene was expressed in E. coli DE3 and was identified by Western blot experiment as the NS gene of WRSV.

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