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Moody DE, Zou Z, McIntyre L. Cross-species hybridisation of pig RNA to human nylon microarrays. BMC Genomics. 2002;3(1):27doi: 10.1186/1471-2164-3-27.
Moody, D. E., Zou, Z., & McIntyre, L. (2002). Cross-species hybridisation of pig RNA to human nylon microarrays. BMC genomics, 3(1), 27. https://doi.org/10.1186/1471-2164-3-27
Moody, D E, et al. "Cross-species hybridisation of pig RNA to human nylon microarrays." BMC genomics vol. 3,1 (2002): 27. doi: https://doi.org/10.1186/1471-2164-3-27
Moody DE, Zou Z, McIntyre L. Cross-species hybridisation of pig RNA to human nylon microarrays. BMC Genomics. 2002 Sep 27;3(1):27. doi: 10.1186/1471-2164-3-27. PMID: 12354330; PMCID: PMC130049.
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BMC Genomics. 2002 Sep 27;3(1):27. doi: 10.1186/1471-2164-3-27.

Cross-species hybridisation of pig RNA to human nylon microarrays.

BMC genomics

D E Moody, Z Zou, L McIntyre

Affiliations

  1. Department of Animal Science, 1151 Lilly Hall, Purdue University, West Lafayette, IN 47907, USA. [email protected]

PMID: 12354330 PMCID: PMC130049 DOI: 10.1186/1471-2164-3-27

Abstract

BACKGROUND: The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed. Three replicate hybridisations of two different amounts of pig RNA, and of human skeletal muscle RNA were completed on three additional microarrays.

RESULTS: Reproducibility of microarray hybridisations of pig cDNA to human microarrays was high, as determined by Spearman and Pearson correlation coefficients and a Kappa statistic. Variability among replicate hybridisations was similar for human and pig data, indicating the reproducibility of results were not compromised in cross-species hybridisations. The concordance between data generated from hybridisations using pig and human skeletal muscle RNA was high, further supporting the use of human microarrays for the analysis of gene expression in the pig. No systematic effect of stripping and re-using nylon microarrays was found, and variability across microarrays was minimal.

CONCLUSION: The majority of genes generated highly reproducible data in cross-species microarray hybridisations, although approximately 6% were identified as highly variable. Experimental designs that include at least three replicate hybridisations for each experimental treatment will enable the variability of individual genes to be considered appropriately. The use of cross-species microarray analysis looks promising. However, additional validation is needed to determine the specificity of cross-species hybridisations, and the validity of results.

References

  1. Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9834-9 - PubMed
  2. Life Sci. 2000 Nov 24;68(1):19-28 - PubMed
  3. Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1176-81 - PubMed
  4. Genome Res. 2001 Apr;11(4):626-30 - PubMed
  5. Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13266-71 - PubMed
  6. Gene. 1999 Jun 11;233(1-2):181-8 - PubMed
  7. Biotechniques. 2002 Mar;32(3):620-5 - PubMed
  8. Science. 1995 Oct 20;270(5235):467-70 - PubMed
  9. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 - PubMed
  10. Science. 1999 Jan 1;283(5398):83-7 - PubMed
  11. Am J Physiol. 1999 Feb;276(2 Pt 2):R308-16 - PubMed
  12. Am J Physiol Heart Circ Physiol. 2002 Feb;282(2):H414-22 - PubMed

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