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Morre DJ, Brightman AO, Barr R, et al. NADH Oxidase Activity of Plasma Membranes of Soybean Hypocotyls Is Activated by Guanine Nucleotides. Plant Physiol. 1993;102(2):595-602doi: 10.1104/pp.102.2.595.
Morre, D. J., Brightman, A. O., Barr, R., Davidson, M., & Crane, F. L. (1993). NADH Oxidase Activity of Plasma Membranes of Soybean Hypocotyls Is Activated by Guanine Nucleotides. Plant physiology, 102(2), 595-602. https://doi.org/10.1104/pp.102.2.595
Morre, D. J., et al. "NADH Oxidase Activity of Plasma Membranes of Soybean Hypocotyls Is Activated by Guanine Nucleotides." Plant physiology vol. 102,2 (1993): 595-602. doi: https://doi.org/10.1104/pp.102.2.595
Morre DJ, Brightman AO, Barr R, Davidson M, Crane FL. NADH Oxidase Activity of Plasma Membranes of Soybean Hypocotyls Is Activated by Guanine Nucleotides. Plant Physiol. 1993 Jun;102(2):595-602. doi: 10.1104/pp.102.2.595. PMID: 12231849; PMCID: PMC158817.
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Plant Physiol. 1993 Jun;102(2):595-602. doi: 10.1104/pp.102.2.595.

NADH Oxidase Activity of Plasma Membranes of Soybean Hypocotyls Is Activated by Guanine Nucleotides.

Plant physiology

D. J. Morre, A. O. Brightman, R. Barr, M. Davidson, F. L. Crane

Affiliations

  1. Department of Medicinal Chemistry (D.J.M., A.O.B., M.D.) and Department of Biological Sciences (R.B., F.L.C.), Purdue University, West Lafayette, Indiana 47907.

PMID: 12231849 PMCID: PMC158817 DOI: 10.1104/pp.102.2.595

Abstract

The activity of an auxin-stimulated NADH oxidase of the plasma membrane of hypocotyls of etiolated soybean (Glycine max Merr.) seedlings responded to guanine and other nucleotides, but in a manner that differed from that of enzymes coupled to the classic trimeric and low molecular weight monomeric guanine nucleotide-binding proteins (G proteins). In the presence and absence of either auxin or divalent ions, both GTP and GDP as well as guanosine-5[prime]-O-(3-thiotriphosphate) (GTP-[gamma]-S) and other nucleoside di- and triphosphates stimulated the oxidase activity over the range 10 [mu]M to 1 mM. GTP and GTP-[gamma]-S stimulated the activity at 10 nM in the absence of added magnesium and at 1 nM in the presence of added magnesium ions. Other nucleotides stimulated at 100 nM and above. The NADH oxidase was stimulated by 10 [mu]M mastoparan and by 40 [mu]M aluminum fluoride. Neither cholera nor pertussis toxins, tested at a concentration sufficient to block mammalian G protein function, inhibited the activity. Guanosine 5[prime]-O-(2-thiodi-phosphate) (GDP-[beta]-S) did not stimulate activity, suggesting that the stimulation in response to GDP may be mediated by a plasma membrane nucleoside diphosphate kinase through conversion of GDP to GTP. Auxin stimulation of the NADH oxidase was unaffected by nucleotides at either high or low nucleotide concentrations in the absence of added divalent ions. However, pretreatment of plasma membranes with auxin increased the apparent affinity for nucleotide binding. This increased affinity, however, appeared not to be the mechanism of auxin stimulation of the oxidase, since auxin stimulation was similar with or without low concentrations of guanine nucleotides. The stimulation by nucleotides was observed after incubating the membranes with 0.1% Triton X-100 prior to assay. The results suggest a role of guanine (and other) nucleotides in the regulation of plasma membrane NADH oxidase that differs from the interactions with G proteins commonly described for animal models.

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