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Kambe-Honjoh H, Ohsumi K, Shimoi H, et al. Molecular breeding of yeast with higher metal-adsorption capacity by expression of histidine-repeat insertion in the protein anchored to the cell wall. J Gen Appl Microbiol. 2000;46(3):113-117doi: 10.2323/jgam.46.113.
Kambe-Honjoh, H., Ohsumi, K., Shimoi, H., Nakajima, H., & Kitamoto, K. (2000). Molecular breeding of yeast with higher metal-adsorption capacity by expression of histidine-repeat insertion in the protein anchored to the cell wall. The Journal of general and applied microbiology, 46(3), 113-117. https://doi.org/10.2323/jgam.46.113
Kambe-Honjoh, Hideko, et al. "Molecular breeding of yeast with higher metal-adsorption capacity by expression of histidine-repeat insertion in the protein anchored to the cell wall." The Journal of general and applied microbiology vol. 46,3 (2000): 113-117. doi: https://doi.org/10.2323/jgam.46.113
Kambe-Honjoh H, Ohsumi K, Shimoi H, Nakajima H, Kitamoto K. Molecular breeding of yeast with higher metal-adsorption capacity by expression of histidine-repeat insertion in the protein anchored to the cell wall. J Gen Appl Microbiol. 2000 Jun;46(3):113-117. doi: 10.2323/jgam.46.113. PMID: 12483584.
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J Gen Appl Microbiol. 2000 Jun;46(3):113-117. doi: 10.2323/jgam.46.113.

Molecular breeding of yeast with higher metal-adsorption capacity by expression of histidine-repeat insertion in the protein anchored to the cell wall.

The Journal of general and applied microbiology

Hideko Kambe-Honjoh, Keisuke Ohsumi, Hitoshi Shimoi, Harushi Nakajima, Katsuhiko Kitamoto

Affiliations

  1. Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

PMID: 12483584 DOI: 10.2323/jgam.46.113

Abstract

A fusion protein of hexa-histidine repeat (His) and glycosylphosphatidylinositol (GPI)-anchor region of Saccharomyces cerevisiae Cwp1 with Aspergillus oryzae Taka-amylase A (TAA) was expressed on the yeast cell surface. The expressed fusion protein (TAA-His-Cwp1) was localized on the cell wall and demonstrated amylolytic activity. In comparison with the TAA-Cwp1 expressing strain, these cells exhibited 1.6- to 2.8-fold higher adsorbing capacity for Cu(2+), Ni(2+), and Zn(2+).

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