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Levy H, Kindle KL, Stern DB. A Nuclear Mutation That Affects the 3[prime] Processing of Several mRNAs in Chlamydomonas Chloroplasts. Plant Cell. 1997;9(5):825-836doi: 10.1105/tpc.9.5.825.
Levy, H., Kindle, K. L., & Stern, D. B. (1997). A Nuclear Mutation That Affects the 3[prime] Processing of Several mRNAs in Chlamydomonas Chloroplasts. The Plant cell, 9(5), 825-836. https://doi.org/10.1105/tpc.9.5.825
Levy, H., et al. "A Nuclear Mutation That Affects the 3[prime] Processing of Several mRNAs in Chlamydomonas Chloroplasts." The Plant cell vol. 9,5 (1997): 825-836. doi: https://doi.org/10.1105/tpc.9.5.825
Levy H, Kindle KL, Stern DB. A Nuclear Mutation That Affects the 3[prime] Processing of Several mRNAs in Chlamydomonas Chloroplasts. Plant Cell. 1997 May;9(5):825-836. doi: 10.1105/tpc.9.5.825. PMID: 12237367; PMCID: PMC156959.
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Plant Cell. 1997 May;9(5):825-836. doi: 10.1105/tpc.9.5.825.

A Nuclear Mutation That Affects the 3[prime] Processing of Several mRNAs in Chlamydomonas Chloroplasts.

The Plant cell

H. Levy, K. L. Kindle, D. B. Stern

Affiliations

  1. Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, New York 14853.

PMID: 12237367 PMCID: PMC156959 DOI: 10.1105/tpc.9.5.825

Abstract

We previously created and analyzed a Chlamydomonas reinhardtii strain, [delta]26, in which an inverted repeat in the 3[prime] untranslated region of the chloroplast atpB gene was deleted. In this strain, atpB transcripts are unstable and heterogeneous in size, and growth is poor under conditions in which photosynthesis is required. Spontaneous suppressor mutations that allow rapid photosynthetic growth have been identified. One strain, [delta]26S, retains the atpB deletion yet accumulates a discrete and stable atpB transcript as a consequence of a recessive nuclear mutation. Unlike previously isolated Chlamydomonas nuclear mutations that affect chloroplast mRNA accumulation, the mutation in [delta]26S affects several chloroplast transcripts. For example, in the atpA gene cluster, the relative abundance of several messages was altered in a manner consistent with inefficient mRNA 3[prime] end processing. Furthermore, [delta]26S cells accumulated novel transcripts with 3[prime] termini in the petD-trnR intergenic region. These transcripts are potential intermediates in 3[prime] end processing. In contrast, no alterations were detected for petD, atpA, or atpB mRNA 5[prime] ends; neither were there gross alterations detected for several other mRNAs, including the wild-type atpB transcript. We suggest that the gene identified by the suppressor mutation encodes a product involved in the processing of monocistronic and polycistronic messages.

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