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Yao HP, Zhang LH, Sun WJ, et al. [Effects of intrasplenic transplantation of IL-18 gene-modified fetal hepatocytes on mouse immune function]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002;31(2):115-120doi: 10.3785/j.issn.1008-9292.2002.02.013.
Yao, H. P., Zhang, L. H., Sun, W. J., & Leng, J. H. (2002). [Effects of intrasplenic transplantation of IL-18 gene-modified fetal hepatocytes on mouse immune function]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 31(2), 115-120. https://doi.org/10.3785/j.issn.1008-9292.2002.02.013
Yao, Hang-Ping, et al. "[Effects of intrasplenic transplantation of IL-18 gene-modified fetal hepatocytes on mouse immune function]." Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences vol. 31,2 (2002): 115-120. doi: https://doi.org/10.3785/j.issn.1008-9292.2002.02.013
Yao HP, Zhang LH, Sun WJ, Leng JH. [Effects of intrasplenic transplantation of IL-18 gene-modified fetal hepatocytes on mouse immune function]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002 Apr;31(2):115-120. doi: 10.3785/j.issn.1008-9292.2002.02.013. Chinese. PMID: 12539273.
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Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002 Apr;31(2):115-120. doi: 10.3785/j.issn.1008-9292.2002.02.013.

[Effects of intrasplenic transplantation of IL-18 gene-modified fetal hepatocytes on mouse immune function].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences

[Article in Chinese]
Hang-Ping Yao, Li-Huang Zhang, Wen-Ji Sun, Jian-Hang Leng

Affiliations

  1. Institute of Immunology, College of Medical Sciences, Zhejiang University, Hangzhou 310031, China.

PMID: 12539273 DOI: 10.3785/j.issn.1008-9292.2002.02.013

Abstract

OBJECTIVE: To investigate the effects of IL-18 gene-modified fetal hepatocytes (AdmIL-18/MNL.CL2) intrasplenic transplantation on mouse immune function. METHODS: Forty mice were evenly divided into 4 groups of 10. Each group received an intrasplenic transplantation one of the following: AdmIL-18/BNL.CL2, Ad-LacZ/BNL.CL2 (virus control), BNL.CL2 (cell control) and PBS (blank control). After two weeks, the mice were sacrificed. Serum cytokine levels, Mpsi and splenic cell culture supernatant and liver tissue extracts supernatants were measured using ELISA. Hepatic cytokines mRNA expression were determined by RT-PCR. THe cytotoxicity of peritoneal Mpsi and NK activity of spienocytes were detected by LDH release assay. The proliferation of splenic lymphocytes was determined by MTT assay. RESULTS: The IL-18, IL-2,IFN-gamma, TNF-alpha levels of serum, Mpsi and splenocyte culture supernatant, liver tissue extracts supernatants in mice transplanted with AdmIL-18/BNL.CL2 were higher and the IL-4, IL-10 levels were lower compared to their levels in other 3 groups. The highest IL-18, IL-2, IFN-gamma, TNF-alpha and the lowest IL-4, IL-10 mRNA expressions in the liver were observed in mice transplanted with AdmIL-18/BNL.CL2. The mice transplanted with AdmIL-18/BNL.Cl2 showed significantly increases cytotoxicity of Mpsi, NK activity and splenic cell proliferation compared with the other 3 groups. CONCLUSION: AdmIL-18 can be effectively transfected into mice fetal heptocytes which subsequently IL-18. Intransplenic transplantation of IL-18 gene-modified fetal hepatocytes may augment mouse immune function and provide an useful basis for targeted gene therapy of liver disease.

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