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Zang WP, Wei XD, Fu S, et al. [Transfer and Expression of Recombinant Human Thrombopoietin Gene in COS-7 Cells and Mice In Vivo]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2001;9(1):14-17
Zang, W. P., Wei, X. D., Fu, S., Wang, S. W., Tang, J., & Wang, D. B. (2001). [Transfer and Expression of Recombinant Human Thrombopoietin Gene in COS-7 Cells and Mice In Vivo]. Zhongguo shi yan xue ye xue za zhi, 9(1), 14-17.
Zang, Wei-Ping, et al. "[Transfer and Expression of Recombinant Human Thrombopoietin Gene in COS-7 Cells and Mice In Vivo]." Zhongguo shi yan xue ye xue za zhi vol. 9,1 (2001): 14-17.
Zang WP, Wei XD, Fu S, Wang SW, Tang J, Wang DB. [Transfer and Expression of Recombinant Human Thrombopoietin Gene in COS-7 Cells and Mice In Vivo]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2001 Mar;9(1):14-17. Chinese. PMID: 12578637.
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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2001 Mar;9(1):14-17.

[Transfer and Expression of Recombinant Human Thrombopoietin Gene in COS-7 Cells and Mice In Vivo].

Zhongguo shi yan xue ye xue za zhi

[Article in Chinese]
Wei-Ping Zang, Xu-Dong Wei, Shuang Fu, Shen-Wu Wang, Jian Tang, De-Bing Wang

Affiliations

  1. Institute of Hematology, People's Hospital, Peking University, Beijing 100044, China; Institute of Cardiovascular Research, Peking University, Beijing 100083, China.

PMID: 12578637

Abstract

In order to investigate the expression of recombinant TPO gene in COS-7 cells and in vivo of the mouse model, eukaryotic expressing plasmid pcd2/TPO with human TPO cDNA was constructed with DNA recombinant techniques. The plasmid pcd2/TPO was transiently transfected into the COS-7 cells by means of lipofection, the naked pcd2/TPO plasmid was injected into the skeletal muscle of mice with electric pulses. RT-PCR and ELISA methods were used to detect the TPO expression of the transfected COS-7 cells, both showed high level expression. The MTT test showed the expressed TPO had proliferative activity to TPO-dependent cell line. High efficiency of gene transfer in transgenic mice was also observed by RT-PCR and immunohistochemical methods. The serum TPO level [(1 185 +/- 264) ng/L] in transgenic mice was quite different compared with the normal mice [(250 +/- 76) ng/L]. All these results provided solid foundations for the research of TPO gene therapy in the future.

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