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Gun'ko VM, Mikhailova IV, Zarko VI, et al. Study of interaction of proteins with fumed silica in aqueous suspensions by adsorption and photon correlation spectroscopy methods. J Colloid Interface Sci. 2003;260(1):56-69doi: 10.1016/s0021-9797(02)00193-5.
Gun'ko, V. M., Mikhailova, I. V., Zarko, V. I., Gerashchenko, I. I., Guzenko, N. V., Janusz, W., Leboda, R., & Chibowski, S. (2003). Study of interaction of proteins with fumed silica in aqueous suspensions by adsorption and photon correlation spectroscopy methods. Journal of colloid and interface science, 260(1), 56-69. https://doi.org/10.1016/s0021-9797(02)00193-5
Gun'ko, V M, et al. "Study of interaction of proteins with fumed silica in aqueous suspensions by adsorption and photon correlation spectroscopy methods." Journal of colloid and interface science vol. 260,1 (2003): 56-69. doi: https://doi.org/10.1016/s0021-9797(02)00193-5
Gun'ko VM, Mikhailova IV, Zarko VI, Gerashchenko II, Guzenko NV, Janusz W, Leboda R, Chibowski S. Study of interaction of proteins with fumed silica in aqueous suspensions by adsorption and photon correlation spectroscopy methods. J Colloid Interface Sci. 2003 Apr 01;260(1):56-69. doi: 10.1016/s0021-9797(02)00193-5. PMID: 12742034.
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J Colloid Interface Sci. 2003 Apr 01;260(1):56-69. doi: 10.1016/s0021-9797(02)00193-5.

Study of interaction of proteins with fumed silica in aqueous suspensions by adsorption and photon correlation spectroscopy methods.

Journal of colloid and interface science

V M Gun'ko, I V Mikhailova, V I Zarko, I I Gerashchenko, N V Guzenko, W Janusz, R Leboda, S Chibowski

Affiliations

  1. Institute of Surface Chemistry, 17 General Naumov Street, 03164 Kiev, Ukraine. [email protected]

PMID: 12742034 DOI: 10.1016/s0021-9797(02)00193-5

Abstract

The interaction of fumed silica A-300 (S(BET) = 297 m2 g(-1)) with bovine serum albumin (prepared by different methods), ovalbumin, human hemoglobin, and gelatin as a function of pH, salinity, and concentrations of components in aqueous medium was studied by adsorption and photon correlation spectroscopy (PCS) methods. Comparison of equilibrium (incubation time t(i) approximately 1 h) adsorption of proteins on A-300, minute (t(i) approximately 1 min) flocculation rate, and the particle size distributions measured by the PCS method shows different rearrangement of particle swarms depending on pH, salinity, and concentration of proteins, especially at pH close to IEP of silica or proteins. The electrokinetic mobility of protein/silica swarms is greater than that of individual components at pH far from the IEP of proteins. Changes in the Gibbs free energy (DeltaG) on protein adsorption depend on pH (-DeltaG is minimal at pH 2, close to the IEP of silica, and maximal at pH between the IEP of protein and silica), concentration (-DeltaG is maximal at C(p) between 1 and 6 mg/ml), type of proteins, and their preparation technique.

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