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Sun D, Steele JE. Regulation of intracellular calcium in dispersed fat body trophocytes of the cockroach, Periplaneta americana, by hypertrehalosemic hormone. J Insect Physiol. 2001;47(12):1399-1408doi: 10.1016/s0022-1910(01)00130-5.
Sun, D., & Steele, J. E. (2001). Regulation of intracellular calcium in dispersed fat body trophocytes of the cockroach, Periplaneta americana, by hypertrehalosemic hormone. Journal of insect physiology, 47(12), 1399-1408. https://doi.org/10.1016/s0022-1910(01)00130-5
Sun, D, and Steele, J E.. "Regulation of intracellular calcium in dispersed fat body trophocytes of the cockroach, Periplaneta americana, by hypertrehalosemic hormone." Journal of insect physiology vol. 47,12 (2001): 1399-1408. doi: https://doi.org/10.1016/s0022-1910(01)00130-5
Sun D, Steele JE. Regulation of intracellular calcium in dispersed fat body trophocytes of the cockroach, Periplaneta americana, by hypertrehalosemic hormone. J Insect Physiol. 2001 Dec;47(12):1399-1408. doi: 10.1016/s0022-1910(01)00130-5. PMID: 12770146.
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J Insect Physiol. 2001 Dec;47(12):1399-1408. doi: 10.1016/s0022-1910(01)00130-5.

Regulation of intracellular calcium in dispersed fat body trophocytes of the cockroach, Periplaneta americana, by hypertrehalosemic hormone.

Journal of insect physiology

D Sun, J E. Steele

Affiliations

  1. Department of Zoology, The University of Western Ontario, Ont., N6A 5B7, London, Canada

PMID: 12770146 DOI: 10.1016/s0022-1910(01)00130-5

Abstract

Incubation of trophocytes from dissaggregated fat body of Periplaneta americana with either of the hypertrehalosemic hormones, HTH-I or HTH-II, leads to an increase in the cytosolic concentration of Ca(2+) from approximately 80 to approximately 310nM with a rise time of approximately 110s. The Ca(2+) concentration then declines to the resting level during the ensuing 5min. In the absence of extracellular Ca(2+) the increase in [Ca(2+)](i) due to HTH is limited to approximately 100nM. The calmodulin inhibitors calmidazolium and W-7 also limit to a similar degree the ability of HTH to increase [Ca(2+)](i). Phorbol 12-myristate 13-acetate, an activator of protein kinase C, was shown to block Ca(2+) entry through the plasma membrane. Additional evidence to support the view that HTH enhances Ca(2+) influx has been obtained by measuring the quenching of fura-2 fluorescence when Ca(2+) is replaced with Mn(2+).

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