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Yamazaki K, Fukuda K, Matsukawa M, et al. Reactive oxygen species depolymerize hyaluronan: involvement of the hydroxyl radical. Pathophysiology. 2003;9(4):215-220doi: 10.1016/s0928-4680(03)00024-5.
Yamazaki, K., Fukuda, K., Matsukawa, M., Hara, F., Yoshida, K., Akagi, M., Munakata, H., & Hamanishi, C. (2003). Reactive oxygen species depolymerize hyaluronan: involvement of the hydroxyl radical. Pathophysiology : the official journal of the International Society for Pathophysiology, 9(4), 215-220. https://doi.org/10.1016/s0928-4680(03)00024-5
Yamazaki, Kenji, et al. "Reactive oxygen species depolymerize hyaluronan: involvement of the hydroxyl radical." Pathophysiology : the official journal of the International Society for Pathophysiology vol. 9,4 (2003): 215-220. doi: https://doi.org/10.1016/s0928-4680(03)00024-5
Yamazaki K, Fukuda K, Matsukawa M, Hara F, Yoshida K, Akagi M, Munakata H, Hamanishi C. Reactive oxygen species depolymerize hyaluronan: involvement of the hydroxyl radical. Pathophysiology. 2003 Sep;9(4):215-220. doi: 10.1016/s0928-4680(03)00024-5. PMID: 14567924.
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Pathophysiology. 2003 Sep;9(4):215-220. doi: 10.1016/s0928-4680(03)00024-5.

Reactive oxygen species depolymerize hyaluronan: involvement of the hydroxyl radical.

Pathophysiology : the official journal of the International Society for Pathophysiology

Kenji Yamazaki, Kanji Fukuda, Masataka Matsukawa, Fumihiko Hara, Koji Yoshida, Masao Akagi, Hiroshi Munakata, Chiaki Hamanishi

Affiliations

  1. Department of Orthopedic Surgery, Kinki University School of Medicine, 377-2 Ohno-higashi, Osaka-sayama, 589-8511, Osaka, Japan

PMID: 14567924 DOI: 10.1016/s0928-4680(03)00024-5

Abstract

We have previously demonstrated that reactive oxygen species (ROS) are involved in cartilage degradation. A decrease in the size of hyaluronan (HA), which is the major macromolecule in synovial fluid and is responsible for imparting viscosity to it, is reported in arthritis patients. The purpose of this study is to determine the ROS that depolymerize HA. The luminol derivative, L-012, was used to determine the generation of ROS. To generate hydroxyl radicals, a mixture of hydrogen peroxide (H(2)O(2)) and ferrous ions (Fe(2+)) was added to HA. The antioxidants and the depolymerization of HA were studied in this system. The hydroxyl radical is one of the ROS, causing the depolymerization of HA, which reacts with L-01. These data suggest that hydroxyl radicals play an important role at the site of inflammation.

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