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Ivan MG, Scaiano JC. A new approach for the detection of carbon-centered radicals in enzymatic processes using prefluorescent probes. Photochem Photobiol. 2003;78(4):416-9doi: 10.1562/0031-8655(2003)078<0416:anaftd>2.0.co;2.
Ivan, M. G., & Scaiano, J. C. (2003). A new approach for the detection of carbon-centered radicals in enzymatic processes using prefluorescent probes. Photochemistry and photobiology, 78(4), 416-9. https://doi.org/10.1562/0031-8655(2003)078<0416:anaftd>2.0.co;2
Ivan, Marius G, and Scaiano, J C. "A new approach for the detection of carbon-centered radicals in enzymatic processes using prefluorescent probes." Photochemistry and photobiology vol. 78,4 (2003): 416-9. doi: https://doi.org/10.1562/0031-8655(2003)078<0416:anaftd>2.0.co;2
Ivan MG, Scaiano JC. A new approach for the detection of carbon-centered radicals in enzymatic processes using prefluorescent probes. Photochem Photobiol. 2003 Oct;78(4):416-9. doi: 10.1562/0031-8655(2003)078<0416:anaftd>2.0.co;2. PMID: 14626672.
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Photochem Photobiol. 2003 Oct;78(4):416-9. doi: 10.1562/0031-8655(2003)078<0416:anaftd>2.0.co;2.

A new approach for the detection of carbon-centered radicals in enzymatic processes using prefluorescent probes.

Photochemistry and photobiology

Marius G Ivan, J C Scaiano

Affiliations

  1. Centre for Catalysis Research and Innovation, Department of Chemistry, University of Ottawa, Ottawa, Ontario, Canada.

PMID: 14626672 DOI: 10.1562/0031-8655(2003)078<0416:anaftd>2.0.co;2

Abstract

In this communication we propose a novel application for prefluorescent probes in the detection of free carbon-centered radicals in enzymatic processes. Prefluorescent probes combine a fluorescent moiety tethered to a paramagnetic nitroxide that acts as a fluorescence quencher. Trapping of a radical by the nitroxide group restores the fluorescence properties. The increase in fluorescence intensity with time reflects the formation and quenching of carbon-centered radicals and can be used for the quantitative evaluation of yields and kinetics. As a test system we used horseradish peroxidase, an oxidoreductase that is widely accepted to operate by a radical-mediated mechanism. We used the prefluorescent probe (quinoline-TEMPO), where a quinoline moiety has been tethered to 2,2,6,6-tetramethylpiperidin-1-oxyl.

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