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Trends Cell Biol. 1993 Jul;3(7):214-9. doi: 10.1016/0962-8924(93)90114-g.

The immunofluorescent era of membrane traffic.

Trends in cell biology

G Griffiths, R G Parton, J Lucocq, B van Deurs, D Brown, J W Slot, H J Geuze

Affiliations

  1. European Molecular Biology Laboratory, Heidelberg, Germany.

PMID: 14731755 DOI: 10.1016/0962-8924(93)90114-g

Abstract

In recent years immunofluorescence microscopy has been increasingly used to study membrane traffic. In this article seven electron microscopists, all with considerable experience in using light microscopy, take a critical look at the immunofluorescence approach and argue that results obtained with this method are often overinterpreted.

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