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Anal Bioanal Chem. 1996 Jun;355(3):364-6. doi: 10.1007/s0021663550364.

Intracavity laser polarization spectrometer for biomolecule traces.

Analytical and bioanalytical chemistry

V S Burakov, A F Lobazov, S V Nechaev, S N Raikov

Affiliations

  1. Institute of Molecular and Atomic Physics, 220072, Minsk, Belarus.

PMID: 15045414 DOI: 10.1007/s0021663550364

Abstract

A polarization variant of an intracavity laser spectrometer based on the competition of beams in an argon ion laser, radiating a number of narrow lines in the 275 to 529 nm spectral range, is proposed and described for the spectral analysis of liquid samples, having broad absorption bands, mainly biomolecules in solutions. By careful optimization of the operating conditions, a procedure has been established that results in a minimal measurable absorbance decreased up to 10(-5) units of optical density. The examined spectrometer provides a wide dynamic range of analysis. A determination of trace contents of amino acids in methanol were carried out.

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