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Mabuchi A, Mullaney I, Sheard P, et al. Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells. Comp Hepatol. 2004;3:S29doi: 10.1186/1476-5926-2-S1-S29.
Mabuchi, A., Mullaney, I., Sheard, P., Hessian, P., Zimmermann, A., Senoo, H., & Wheatley, A. M. (2004). Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells. Comparative hepatology, 3S29. https://doi.org/10.1186/1476-5926-2-S1-S29
Mabuchi, Ayako, et al. "Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells." Comparative hepatology vol. 3 (2004): S29. doi: https://doi.org/10.1186/1476-5926-2-S1-S29
Mabuchi A, Mullaney I, Sheard P, Hessian P, Zimmermann A, Senoo H, Wheatley AM. Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells. Comp Hepatol. 2004 Jan 14;3:S29. doi: 10.1186/1476-5926-2-S1-S29. PMID: 14960181; PMCID: PMC2410248.
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Comp Hepatol. 2004 Jan 14;3:S29. doi: 10.1186/1476-5926-2-S1-S29.

Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells.

Comparative hepatology

Ayako Mabuchi, Ian Mullaney, Philip Sheard, Paul Hessian, Arthur Zimmermann, Haruki Senoo, Antony M Wheatley

Affiliations

  1. Department of Physiology, University of Otago, Dunedin, New Zealand. [email protected]

PMID: 14960181 PMCID: PMC2410248 DOI: 10.1186/1476-5926-2-S1-S29

Abstract

We investigated activation mechanisms of hepatic stellate cells (HSCs) that are known to play pivotal roles in the regeneration process after 70% partial hepatectomy (PHx). Parenchymal liver cells (PLCs) and non-parenchymal cells (NPLCs) were isolated and purified from the regenerating livers at 1, 3, 7, 14 days after PHx. Each liver cell fraction was stained by immunocytochemistry using an anti-desmin antibody as a marker for HSCs, anti-alpha-smooth muscle actin (alpha-SMA) as a marker for activated HSCs, and 5-bromo-2'-deoxyuridine (BrdU) for detection of proliferating cells. Tissue sections from regenerating livers were also analyzed by immunohistochemistry and compared with the results obtained for isolated cell fractions. One and 3 days after PHx, PLC-enriched fraction contained HSCs adhered to PLCs. The HSCs adhered to PLCs were double positive for BrdU and alpha-SMA, and formed clusters suggesting that these HSCs were activated. However, HSC-enriched fraction contained HSCs not adhered PLCs showed positive staining for anti-desmin antibody but negative for anti-alpha-SMA antibody. These results suggest that HSCs are activated by adhering to PLCs during the early phase of hepatic regeneration.

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