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Ma P, Sivaloganathan B, Reddy PK, et al. Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer). Mar Biotechnol (NY). 2001;3(5):463-9doi: 10.1007/s10126-001-0018-1.
Ma, P., Sivaloganathan, B., Reddy, P. K., Chan, W. K., & Lam, T. J. (2001). Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer). Marine biotechnology (New York, N.Y.), 3(5), 463-9. https://doi.org/10.1007/s10126-001-0018-1
Ma, P, et al. "Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer)." Marine biotechnology (New York, N.Y.) vol. 3,5 (2001): 463-9. doi: https://doi.org/10.1007/s10126-001-0018-1
Ma P, Sivaloganathan B, Reddy PK, Chan WK, Lam TJ. Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer). Mar Biotechnol (NY). 2001 Sep;3(5):463-9. doi: 10.1007/s10126-001-0018-1. PMID: 14961339.
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Mar Biotechnol (NY). 2001 Sep;3(5):463-9. doi: 10.1007/s10126-001-0018-1.

Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer).

Marine biotechnology (New York, N.Y.)

P Ma, B Sivaloganathan, P K Reddy, W K Chan, T J Lam

Affiliations

  1. Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.

PMID: 14961339 DOI: 10.1007/s10126-001-0018-1

Abstract

The enzymatic activities of alpha-amylase and its corresponding messenger RNA levels in developing sea bass (Lates calcarifer) larvae were studied from hatching until 27 days post hatching (dph). An increasing activity of amylase enzyme was measured until 5 dph, and the activity gradually decreased thereafter and reached a constant level by 12 dph. To achieve a better understanding of the molecular mechanisms underlying amylase expression, we have cloned and sequenced a 318-bp fragment of alpha-amylase complementary DNA. Based on this sequence, a real-time reverse transcriptase polymerase chain reaction technique to monitor the changes in the mRNA levels in the larvae was developed. A correlation between enzymatic activity and mRNA level of alpha-amylase could be demonstrated during the early development of sea bass larvae. This suggests that the changes in alpha-amylase are controlled at least at the transcriptional level during early larval development of sea bass.

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