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Pitkänen TI, Xie SQ, Krasnov A, et al. Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene. Mar Biotechnol (NY). 2001;3(2):188-97doi: 10.1007/s101260000060.
Pitkänen, T. I., Xie, S. Q., Krasnov, A., Mason, P. S., Mölsä, H., & Stickland, N. C. (2001). Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene. Marine biotechnology (New York, N.Y.), 3(2), 188-97. https://doi.org/10.1007/s101260000060
Pitkänen, T I, et al. "Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene." Marine biotechnology (New York, N.Y.) vol. 3,2 (2001): 188-97. doi: https://doi.org/10.1007/s101260000060
Pitkänen TI, Xie SQ, Krasnov A, Mason PS, Mölsä H, Stickland NC. Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene. Mar Biotechnol (NY). 2001 Mar;3(2):188-97. doi: 10.1007/s101260000060. PMID: 14961382.
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Mar Biotechnol (NY). 2001 Mar;3(2):188-97. doi: 10.1007/s101260000060.

Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene.

Marine biotechnology (New York, N.Y.)

T I Pitkänen, S Q Xie, A Krasnov, P S Mason, H Mölsä, N C Stickland

Affiliations

  1. University of Kuopio, Institute of Applied Biotechnology, P.O. Box 1627, 70211 Kuopio, Finland.

PMID: 14961382 DOI: 10.1007/s101260000060

Abstract

Biochemical and histological analyses were used to study the number and size of cells (cellularity) in tissues of fast-growing, genetically modified Arctic char (Salvelinus alpinus L.), overexpressing sockeye salmon (Oncorhynchus nerka) growth hormone gene (OnGH1). DNA contents of muscle, heart, and liver were compared in transformed, sibling (age control) and 1 year older (size control) char. Total white muscle cross-sectional area, white muscle fiber number, and total nuclei number within the muscle tissue were determined from one complete half-section of each fish. The analyzed tissues responded differently to growth hormone overproduction. In muscle tissue of OnGH1-transformed char, the enhanced growth was clearly associated with proliferation of muscle cells (hyperplasia), whereas in heart tissue both cell proliferation and increase in cell size (hypertrophy) were enhanced. The relative DNA concentration in the liver of transformed char was significantly greater than that of control fish, suggesting reduction in size of hepatic cells.

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