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Lima HC, Bleyenberg JA, Titus RG. A simple method for quantifying Leishmania in tissues of infected animals. Parasitol Today. 1997;13(2):80-2doi: 10.1016/s0169-4758(96)40010-2.
Lima, H. C., Bleyenberg, J. A., & Titus, R. G. (1997). A simple method for quantifying Leishmania in tissues of infected animals. Parasitology today (Personal ed.), 13(2), 80-2. https://doi.org/10.1016/s0169-4758(96)40010-2
Lima, H C, et al. "A simple method for quantifying Leishmania in tissues of infected animals." Parasitology today (Personal ed.) vol. 13,2 (1997): 80-2. doi: https://doi.org/10.1016/s0169-4758(96)40010-2
Lima HC, Bleyenberg JA, Titus RG. A simple method for quantifying Leishmania in tissues of infected animals. Parasitol Today. 1997 Feb;13(2):80-2. doi: 10.1016/s0169-4758(96)40010-2. PMID: 15275128.
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Parasitol Today. 1997 Feb;13(2):80-2. doi: 10.1016/s0169-4758(96)40010-2.

A simple method for quantifying Leishmania in tissues of infected animals.

Parasitology today (Personal ed.)

H C Lima, J A Bleyenberg, R G Titus

Affiliations

  1. Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1671 USA.

PMID: 15275128 DOI: 10.1016/s0169-4758(96)40010-2

Abstract

In experimental animals infected with Leishmania major, the size of cutaneous lesions of the parasite often does not correlate with the number of parasites within the lesion. Indeed, cutaneous lesions can heal, but still contain parasites. Thus, the ability to determine parasite burden in infected animals becomes important, especially when assessing vaccines that are intended to induce sterilizing immunity. Here, Hermenio Lima, Julie Bleyenberg and Richard Titus describe a simple technique for enumerating Leishmania in infected tissue. It is hoped that this technique will allow all researchers working with Leishmania (especially those in countries where leishmaniasis is endemic) to determine parasite burden easily in infected animals.

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