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Lammie PJ, Weil G, Noordin R, et al. Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial. Filaria J. 2004;3(1):9doi: 10.1186/1475-2883-3-9.
Lammie, P. J., Weil, G., Noordin, R., Kaliraj, P., Steel, C., Goodman, D., Lakshmikanthan, V. B., & Ottesen, E. (2004). Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial. Filaria journal, 3(1), 9. https://doi.org/10.1186/1475-2883-3-9
Lammie, Patrick J, et al. "Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial." Filaria journal vol. 3,1 (2004): 9. doi: https://doi.org/10.1186/1475-2883-3-9
Lammie PJ, Weil G, Noordin R, Kaliraj P, Steel C, Goodman D, Lakshmikanthan VB, Ottesen E. Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial. Filaria J. 2004 Sep 03;3(1):9. doi: 10.1186/1475-2883-3-9. PMID: 15347425; PMCID: PMC519021.
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Filaria J. 2004 Sep 03;3(1):9. doi: 10.1186/1475-2883-3-9.

Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial.

Filaria journal

Patrick J Lammie, Gary Weil, Rahmah Noordin, Perumal Kaliraj, Cathy Steel, David Goodman, Vijaya B Lakshmikanthan, Eric Ottesen

Affiliations

  1. Division of Parasitic Diseases, Centers for Disease Control and Prevention, MS-F13, 4770 Buford Highway, Atlanta, Georgia, 30341, USA. [email protected]

PMID: 15347425 PMCID: PMC519021 DOI: 10.1186/1475-2883-3-9

Abstract

The development of antifilarial antibody responses is a characteristic feature of infection with filarial parasites. It should be possible to exploit this fact to develop tools to monitor the progress of the global program to eliminate lymphatic filariasis (LF); however, assays based on parasite extracts suffer from a number of limitations, including the paucity of parasite material, the difficulty of assay standardization and problems with assay specificity. In principle, assays based on recombinant filarial antigens should address these limitations and provide useful tools for diagnosis and surveillance of LF. The present multicenter study was designed to compare the performance of antibody assays for filariasis based on recombinant antigens Bm14, WbSXP, and BmR1. Coded serum specimens were distributed to five participating laboratories where assays for each antigen were conducted in parallel. Assays based on Bm14, WbSXP, or BmR1 demonstrated good sensitivity (>90%) for field use and none of the assays demonstrated reactivity with specimens from persons with non-filarial helminth infections. Limitations of the assays are discussed. Well-designed field studies are now needed to assess sampling methodology and the application of antibody testing to the monitoring and surveillance of LF elimination programs.

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