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Völcker N, Klee D, Höcker H, et al. Functionalization of silicone rubber for the covalent immobilization of fibronectin. J Mater Sci Mater Med. 2001;12(2):111-9doi: 10.1023/a:1008938525489.
Völcker, N., Klee, D., Höcker, H., & Langefeld, S. (2001). Functionalization of silicone rubber for the covalent immobilization of fibronectin. Journal of materials science. Materials in medicine, 12(2), 111-9. https://doi.org/10.1023/a:1008938525489
Völcker, N, et al. "Functionalization of silicone rubber for the covalent immobilization of fibronectin." Journal of materials science. Materials in medicine vol. 12,2 (2001): 111-9. doi: https://doi.org/10.1023/a:1008938525489
Völcker N, Klee D, Höcker H, Langefeld S. Functionalization of silicone rubber for the covalent immobilization of fibronectin. J Mater Sci Mater Med. 2001 Feb;12(2):111-9. doi: 10.1023/a:1008938525489. PMID: 15348316.
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J Mater Sci Mater Med. 2001 Feb;12(2):111-9. doi: 10.1023/a:1008938525489.

Functionalization of silicone rubber for the covalent immobilization of fibronectin.

Journal of materials science. Materials in medicine

N Völcker, D Klee, H Höcker, S Langefeld

Affiliations

  1. Department of Textile and Macromolecular Chemistry, Technical University of Aachen, Veltmanplatz 8, D-52062 Aachen, Germany. [email protected]

PMID: 15348316 DOI: 10.1023/a:1008938525489

Abstract

Surface modification techniques were employed in order to provide functionalized silicone rubber with enhanced cytocompatibility. Acrylic acid (AAc), methacrylic acid (MAAc) and glycidylmethacrylate (GMA) were graft-co-polymerized onto the surface of silicone induced by an argon plasma and thermal initiation. The polymerizations were carried out in solution, in the case of acrylic acid a vapor phase graft-co-polymerization subsequent to argon plasma activation was carried out as well. Human fibronectin (hFn), which acts as a cell adhesion mediator for fibroblasts, was immobilized by making use of the generated carboxylic or epoxy groups, respectively. Surface analysis was accomplished by means of X-ray photoelectron spectroscopy (XPS), infrared spectroscopy in attenuated total reflection mode (IR-ATR), scanning electron microscopy (SEM), atomic force microscopy (AFM) and dynamic contact angle measurements using the Wilhelmy-plate method. The amount of immobilized active hFn was semiquantified by enzyme-linked immunosorbent assay (ELISA) using a structure-specific antibody against the cell-binding domain of hFn. In vitro testing showed a remarkable difference between surfaces exposing adsorbed-only and surfaces with covalently immobilized hFn.

Copyright 2001 Kluwer Academic Publishers

References

  1. J Biomater Sci Polym Ed. 1993;5(3):205-20 - PubMed
  2. Annu Rev Med. 1984;35:561-75 - PubMed
  3. J Biomed Mater Res. 1992 Dec;26(12):1569-81 - PubMed
  4. Cornea. 1994 Sep;13(5):422-8 - PubMed
  5. Biomaterials. 1995 Sep;16(14):1057-67 - PubMed
  6. Biomaterials. 1994 Feb;15(3):163-71 - PubMed
  7. Biomaterials. 1993 Sep;14(11):817-22 - PubMed
  8. Ophthalmologe. 1992 Apr;89(2):109-18 - PubMed
  9. Biomaterials. 1991 Jul;12(5):521-4 - PubMed
  10. Artif Organs. 1992 Feb;16(1):43-9 - PubMed

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