Biol Chem. 2004 Sep;385(9):853-61. doi: 10.1515/BC.2004.111.

Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry.

Biological chemistry

Sergey Strelkov, Mirko von Elstermann, Dietmar Schomburg

PMID: 15493881 DOI: 10.1515/BC.2004.111

Abstract

An analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation of Corynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation of C. glutamicum to different growth conditions is demonstrated.

MeSH terms

• Corynebacterium glutamicum / chemistry
• Corynebacterium glutamicum / metabolism
• Gas Chromatography-Mass Spectrometry / methods

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't