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Acta Crystallogr D Biol Crystallogr. 1993 May 01;49:352-4. doi: 10.1107/S0907444993000952.

Crystallization of a Humicola lanuginosa lipase-inhibitor complex with the use of polyethylene glycol monomethyl ether.

Acta crystallographica. Section D, Biological crystallography

A M Brzozowski

Affiliations

  1. Department of Chemistry, University of York, Heslington, England.

PMID: 15299525 DOI: 10.1107/S0907444993000952

Abstract

The fungal Humicola lanuginosa lipase complexed with the inhibitor n-dodecylphosphonate ethyl ester was crystallized in space group P2(1)2(1)2(1) with pseudotetragonal unit-cell parameters of a = 131.7 (2), b = 131.3 (1), c = 75.4 (1) A. 92% of X-ray diffraction data to 2.8 A resolution were collected with a final R(merge) = 8.5%. The crystals were grown using a new kind of precipitant - polyethylene glycol monomethyl ether (Peg-mme) of molecular weight 5000.

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