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Luft JR, Albright DT, Baird JK, et al. The rate of water equilibration in vapor-diffusion crystallizations: dependence on the distance from the droplet to the reservoir. Acta Crystallogr D Biol Crystallogr. 1996;52:1098-106doi: 10.1107/S0907444996006932.
Luft, J. R., Albright, D. T., Baird, J. K., & DeTitta, G. T. (1996). The rate of water equilibration in vapor-diffusion crystallizations: dependence on the distance from the droplet to the reservoir. Acta crystallographica. Section D, Biological crystallography, 521098-106. https://doi.org/10.1107/S0907444996006932
Luft, J R, et al. "The rate of water equilibration in vapor-diffusion crystallizations: dependence on the distance from the droplet to the reservoir." Acta crystallographica. Section D, Biological crystallography vol. 52 (1996): 1098-106. doi: https://doi.org/10.1107/S0907444996006932
Luft JR, Albright DT, Baird JK, DeTitta GT. The rate of water equilibration in vapor-diffusion crystallizations: dependence on the distance from the droplet to the reservoir. Acta Crystallogr D Biol Crystallogr. 1996 Nov 01;52:1098-106. doi: 10.1107/S0907444996006932. PMID: 15299569.
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Acta Crystallogr D Biol Crystallogr. 1996 Nov 01;52:1098-106. doi: 10.1107/S0907444996006932.

The rate of water equilibration in vapor-diffusion crystallizations: dependence on the distance from the droplet to the reservoir.

Acta crystallographica. Section D, Biological crystallography

J R Luft, D T Albright, J K Baird, G T DeTitta

Affiliations

  1. Hauptman-Woodward Medical Research Institute, Inc., Buffalo, New York 14203-1196, USA.

PMID: 15299569 DOI: 10.1107/S0907444996006932

Abstract

The rate of water equilibration in hanging-drop vapor-diffusion experiments was studied as a function of the distance separating the hanging drop from the surface of the reservoir solution. Hanging drops of 1.00 M NaCl were allowed to partially equilibrate with reservoirs of 2.00 M NaCl at room temperature. Over the range of droplet-reservoir distances examined, 7.6-119.4 mm, the larger the distance that separated the droplet and reservoir, the slower the droplet equilibrated with the reservoir. The variation of the rate of equilibration with droplet-reservoir distance was non-linear; the rate was most sensitive to variations in the droplet-reservoir separation at short separations. A mathematical model of the equilibration kinetics was developed that fits the experimental data. The model is based on the assumption that the rate-limiting step in vapor-diffusion equilibration is transit of water across the vapor space. A simple device to vary the rate of water equilibration, and thereby optimize macromolecular crystal growth conditions, is described.

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