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Yang XJ, Drepper F, Wu B, et al. From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [RuII(bipy)(terpy)L]2+ complexes (bipy = 2,2'-bipyridine; terpy = 2,2':6',2''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c). Dalton Trans. 2004;(2):256-67doi: 10.1039/b414999h.
Yang, X. J., Drepper, F., Wu, B., Sun, W. H., Haehnel, W., & Janiak, C. (2005). From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [RuII(bipy)(terpy)L]2+ complexes (bipy = 2,2'-bipyridine; terpy = 2,2':6',2''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c). Dalton transactions (Cambridge, England : 2003), (2), 256-67. https://doi.org/10.1039/b414999h
Yang, Xiao-Juan, et al. "From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [RuII(bipy)(terpy)L]2+ complexes (bipy = 2,2'-bipyridine; terpy = 2,2':6',2''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c)." Dalton transactions (Cambridge, England : 2003) vol. ,2 (2005): 256-67. doi: https://doi.org/10.1039/b414999h
Yang XJ, Drepper F, Wu B, Sun WH, Haehnel W, Janiak C. From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [RuII(bipy)(terpy)L]2+ complexes (bipy = 2,2'-bipyridine; terpy = 2,2':6',2''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c). Dalton Trans. 2005 Jan 21;(2):256-67. doi: 10.1039/b414999h. Epub 2004 Dec 09. PMID: 15616713.
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Dalton Trans. 2005 Jan 21;(2):256-67. doi: 10.1039/b414999h. Epub 2004 Dec 09.

From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [RuII(bipy)(terpy)L]2+ complexes (bipy = 2,2'-bipyridine; terpy = 2,2':6',2''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c).

Dalton transactions (Cambridge, England : 2003)

Xiao-Juan Yang, Friedrich Drepper, Biao Wu, Wen-Hua Sun, Wolfgang Haehnel, Christoph Janiak

Affiliations

  1. Institut fur Anorganische und Analytische Chemie, Universitat Freiburg, Albertstr. 21, 79104, Freiburg, Germany.

PMID: 15616713 DOI: 10.1039/b414999h

Abstract

Compounds [RuII(bipy)(terpy)L](PF6)2 with bipy = 2,2'-bipyridine, terpy = 2,2':6',2"-terpyridine, L = H2O, imidazole (imi), 4-methylimidazole, 2-methylimidazole, benzimidazole, 4,5-diphenylimidazole, indazole, pyrazole, 3-methylpyrazole have been synthesized and characterized by 1H NMR, ESI-MS and UV/Vis (in CH3CN and H2O). For L = H2O, imidazole, 4,5-diphenylimidazole and indazole the X-ray structures of the complexes have been determined with the crystal packing featuring only few intermolecular C-H...pi or pi-pi interactions due to the separating action of the PF6-anions. Complexes with L = imidazole and 4-methylimidazole exhibit a fluorescence emission with a maximum at 662 and 667 nm, respectively (lambdaexc= 475 nm, solvent CH3CN or H2O). The substitution of the aqua ligand in [Ru(bipy)(terpy)(H2O)]2+ in aqueous solution by imidazole to give [Ru(bipy)(terpy)(imi)]2+ is fastest at a pH of 8.5 (as followed by the increase in emission intensity). Coupling of the [Ru(bipy)(terpy)]2+ fragment to cytochrome c(Yeast iso-1) starting from the Ru-aqua complex was successful at 35 degrees C and pH 7.0 after 5 d under argon in the dark. The [Ru(bipy)(terpy)(cyt c)]-product was characterized by UV/Vis, emission and mass spectrometry. The location where the [Ru(bipy)(terpy)] complex was coupled to the protein was identified as His44 (corresponding to His39 in other numbering schemes) using digestion of the Ru-coupled protein by trypsin and analysis of the tryptic peptides by HPLC-high resolution MS.

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