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Rémigy HW, Aivaliotis M, Ioannidis N, et al. Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum. Photosynth Res. 2003;78(2):153-60doi: 10.1023/B:PRES.0000004347.34228.2c.
Rémigy, H. W., Aivaliotis, M., Ioannidis, N., Jenö, P., Mini, T., Engel, A., Jaquinod, M., & Tsiotis, G. (2003). Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum. Photosynthesis research, 78(2), 153-60. https://doi.org/10.1023/B:PRES.0000004347.34228.2c
Rémigy, Hervé-W, et al. "Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum." Photosynthesis research vol. 78,2 (2003): 153-60. doi: https://doi.org/10.1023/B:PRES.0000004347.34228.2c
Rémigy HW, Aivaliotis M, Ioannidis N, Jenö P, Mini T, Engel A, Jaquinod M, Tsiotis G. Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum. Photosynth Res. 2003;78(2):153-60. doi: 10.1023/B:PRES.0000004347.34228.2c. PMID: 16245046.
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Photosynth Res. 2003;78(2):153-60. doi: 10.1023/B:PRES.0000004347.34228.2c.

Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum.

Photosynthesis research

Hervé-W Rémigy, Michalis Aivaliotis, Nikos Ioannidis, Paul Jenö, Thierry Mini, Andreas Engel, Michel Jaquinod, Georgios Tsiotis

Affiliations

  1. Division of Biochemistry, Department of Chemistry, University of Crete, 71409, Heraklion, Greece.

PMID: 16245046 DOI: 10.1023/B:PRES.0000004347.34228.2c

Abstract

Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.

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