Display options
Cite
Fiedor L, Stasiek M, Myśliwa-Kurdziel B, et al. Phytol as one of the determinants of chlorophyll interactions in solution. Photosynth Res. 2003;78(1):47-57doi: 10.1023/A:1026042005536.
Fiedor, L., Stasiek, M., Myśliwa-Kurdziel, B., & Strzałka, K. (2003). Phytol as one of the determinants of chlorophyll interactions in solution. Photosynthesis research, 78(1), 47-57. https://doi.org/10.1023/A:1026042005536
Fiedor, Leszek, et al. "Phytol as one of the determinants of chlorophyll interactions in solution." Photosynthesis research vol. 78,1 (2003): 47-57. doi: https://doi.org/10.1023/A:1026042005536
Fiedor L, Stasiek M, Myśliwa-Kurdziel B, Strzałka K. Phytol as one of the determinants of chlorophyll interactions in solution. Photosynth Res. 2003;78(1):47-57. doi: 10.1023/A:1026042005536. PMID: 16245063.
Copy Download .nbib Format: NLM
Share it on

Photosynth Res. 2003;78(1):47-57. doi: 10.1023/A:1026042005536.

Phytol as one of the determinants of chlorophyll interactions in solution.

Photosynthesis research

Leszek Fiedor, Maria Stasiek, Beata Myśliwa-Kurdziel, Kazimierz Strzałka

Affiliations

  1. Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Cracow, Poland, [email protected].

PMID: 16245063 DOI: 10.1023/A:1026042005536

Abstract

Optical absorption and fluorescence parameters of chlorophyll a and the phytol-free chlorophyllide a, as well as of their Mg-depleted derivatives, were compared in a series of organic solvents. In contrast to prevailing opinion, the spectral properties of chlorophyll are not indifferent to the removal of phytol. The electronic absorption spectra of chlorophyll a and chlorophyllide a differ and display a different dependence on the nature of the solvent, which cannot be explained solely by the location of a charged carboxylic group in the proximity of the pi- electron system. In fact, measurements in media of varying basicity show that deprotonation of the free carboxylic group in chlorophyllide, i.e., the presence of a negative point charge near the macrocycle, has no effect on pigment absorption spectra. Analysis of the solvent effect on the Q(Y) energies in terms of solvent polarity reveals that the phytyl moiety perturbs the spectral features of chlorophyll, mainly due to its interactions with the pigment solvation shell. The phytyl residue might also be thus partly involved in controlling the central metal ligation in chlorophylls. This influence of phytol on the spectral features of chlorophyll should be taken into account when comparing the spectra in solution with various spectral forms of chlorophyll in vivo.

References

  1. Biochemistry. 1995 May 9;34(18):6142-52 - PubMed
  2. Arch Microbiol. 1996 Sep;166(3):151-9 - PubMed
  3. Cell Mol Life Sci. 1999 Oct 15;56(3-4):330-47 - PubMed
  4. Structure. 1996 May 15;4(5):581-97 - PubMed
  5. Science. 1996 Jun 21;272(5269):1788-91 - PubMed
  6. Exp Gerontol. 2000 Mar;35(2):145-58 - PubMed
  7. J Biol Chem. 1992 Nov 5;267(31):22043-7 - PubMed
  8. Biophys J. 1984 Oct;46(4):463-77 - PubMed
  9. FEBS Lett. 1991 Nov 18;293(1-2):131-3 - PubMed
  10. Structure. 1996 Apr 15;4(4):449-62 - PubMed
  11. Photochem Photobiol. 1993 Oct;58(4):506-11 - PubMed
  12. Biochemistry. 1996 May 28;35(21):6612-9 - PubMed
  13. Photochem Photobiol. 1996 Jul;64(1):174-81 - PubMed
  14. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7124-8 - PubMed
  15. Biophys Chem. 2000 May 15;84(3):189-94 - PubMed
  16. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4959-62 - PubMed
  17. Photochem Photobiol. 1987 Nov;46(5):639-43 - PubMed
  18. Annu Rev Biophys Bioeng. 1984;13:105-24 - PubMed
  19. Photochem Photobiol. 2000 Jan;71(1):71-83 - PubMed

Publication Types