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J Biosci Bioeng. 2000;89(5):489-91. doi: 10.1016/s1389-1723(00)89102-1.

Isolation and characterization of a tetrachloroethylene dechlorinating bacterium, Clostridium bifermentans DPH-1.

Journal of bioscience and bioengineering

Y C Chang, M Hatsu, K Jung, Y S Yoo, K Takamizawa

Affiliations

  1. Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

PMID: 16232783 DOI: 10.1016/s1389-1723(00)89102-1

Abstract

A tetrachloroethylene (PCE)-degrading gram-positive, endospore forming, anaerobic bacterium, strain DPH-1, was isolated from a contaminated site. The organism was identified as Clostridium bifermentans by 16S rRNA gene sequence analysis and based on its physiological characteristics. Strain DPH-1 could dechlorinate high concentrations of PCE (0.9 mM), via trichloroethylene (TCE) to cis-1,2-dichloroethylene (cDCE) at a rate of 0.43 micromol/h.mg protein, as well as a number of other halogenated aliphatic compounds.

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