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Tanaka H, Kamogawa T, Aoyagi H, et al. Invertase production by Saccharomyces cerevisiae protoplasts immobilized in strontium alginate gel beads. J Biosci Bioeng. 2000;89(5):498-500doi: 10.1016/s1389-1723(00)89105-7.
Tanaka, H., Kamogawa, T., Aoyagi, H., Kato, I., & Nakajima, R. (2000). Invertase production by Saccharomyces cerevisiae protoplasts immobilized in strontium alginate gel beads. Journal of bioscience and bioengineering, 89(5), 498-500. https://doi.org/10.1016/s1389-1723(00)89105-7
Tanaka, H, et al. "Invertase production by Saccharomyces cerevisiae protoplasts immobilized in strontium alginate gel beads." Journal of bioscience and bioengineering vol. 89,5 (2000): 498-500. doi: https://doi.org/10.1016/s1389-1723(00)89105-7
Tanaka H, Kamogawa T, Aoyagi H, Kato I, Nakajima R. Invertase production by Saccharomyces cerevisiae protoplasts immobilized in strontium alginate gel beads. J Biosci Bioeng. 2000;89(5):498-500. doi: 10.1016/s1389-1723(00)89105-7. PMID: 16232786.
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J Biosci Bioeng. 2000;89(5):498-500. doi: 10.1016/s1389-1723(00)89105-7.

Invertase production by Saccharomyces cerevisiae protoplasts immobilized in strontium alginate gel beads.

Journal of bioscience and bioengineering

H Tanaka, T Kamogawa, H Aoyagi, I Kato, R Nakajima

Affiliations

  1. Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.

PMID: 16232786 DOI: 10.1016/s1389-1723(00)89105-7

Abstract

The invertase productivity of Saccharomyces cerevisiae IFO 0309 protoplasts in a static culture was 35 times (extracellular) and 9 times (extracellular + intracellular) higher than those of cells. When S. cerevisiae protoplasts were immobilized in 1.0% strontium alginate gel as an artificial cell wall, the protoplasts could be cultivated in a shake flask without disruption, and invertase was secreted into the broth. However, cell wall regeneration in the immobilized protoplasts was detected at about 24 h of cultivation. This implies that prevention of cell wall regeneration is a prerequisite for long term process with protoplasts. When 0.5 microg/ml aculeacin A (inhibitor of beta-1,3 glucan synthesis) was added to the broth, active protoplasts were maintained without cell wall regeneration for more than 24 h and invertase was produced extracellularly. Immobilized S. cerevisiae T7 protoplasts (this strain produces twice as much invertase as S. cerevisiae IFO 0309) were used for invertase production in a bubble column reactor, and a high and stable level of invertase (45 U/ml) was maintained for 72 h.

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