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Takahashi T, Nakakita Y, Watari J, et al. A new rapid technique for detection of microorganisms using bioluminescence and fluorescence microscope method. J Biosci Bioeng. 2000;89(5):509-13doi: 10.1016/s1389-1723(00)89108-2.
Takahashi, T., Nakakita, Y., Watari, J., & Shinotsuka, K. (2000). A new rapid technique for detection of microorganisms using bioluminescence and fluorescence microscope method. Journal of bioscience and bioengineering, 89(5), 509-13. https://doi.org/10.1016/s1389-1723(00)89108-2
Takahashi, T, et al. "A new rapid technique for detection of microorganisms using bioluminescence and fluorescence microscope method." Journal of bioscience and bioengineering vol. 89,5 (2000): 509-13. doi: https://doi.org/10.1016/s1389-1723(00)89108-2
Takahashi T, Nakakita Y, Watari J, Shinotsuka K. A new rapid technique for detection of microorganisms using bioluminescence and fluorescence microscope method. J Biosci Bioeng. 2000;89(5):509-13. doi: 10.1016/s1389-1723(00)89108-2. PMID: 16232789.
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J Biosci Bioeng. 2000;89(5):509-13. doi: 10.1016/s1389-1723(00)89108-2.

A new rapid technique for detection of microorganisms using bioluminescence and fluorescence microscope method.

Journal of bioscience and bioengineering

T Takahashi, Y Nakakita, J Watari, K Shinotsuka

Affiliations

  1. Brewing Research Laboratories, Sapporo Breweries Ltd., 10 Okatome, Yaizu, Shizuoka 425-0013, Japan.

PMID: 16232789 DOI: 10.1016/s1389-1723(00)89108-2

Abstract

The MicroStar-RMDS-SPS (RMDS; R apid M icrobe D etection S ystem) enables detection and determination of the number of microorganisms trapped on a membrane filter based on the ATP-bioluminescence method. However, this system could not provide information about the identity of contaminating species based on the measurement results. Therefore, we developed a new technique for observing microcolonies of contaminants using a fluorescence microscope (Micro Scanner). The coordinates in the image data of the RMDS were converted to the coordinates of the sample stage of the microscope, and a microcolony could be easily observed. A single yeast cell and a microcolony consisting of 20-30 lactic acid bacterial cells could be observed on a membrane filter after measurement using the RMDS.

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