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Kahar P, Iwata T, Hiraki J, et al. Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control. J Biosci Bioeng. 2001;91(2):190-4doi: 10.1263/jbb.91.190.
Kahar, P., Iwata, T., Hiraki, J., Park, E. Y., & Okabe, M. (2001). Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control. Journal of bioscience and bioengineering, 91(2), 190-4. https://doi.org/10.1263/jbb.91.190
Kahar, P, et al. "Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control." Journal of bioscience and bioengineering vol. 91,2 (2001): 190-4. doi: https://doi.org/10.1263/jbb.91.190
Kahar P, Iwata T, Hiraki J, Park EY, Okabe M. Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control. J Biosci Bioeng. 2001;91(2):190-4. doi: 10.1263/jbb.91.190. PMID: 16232973.
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J Biosci Bioeng. 2001;91(2):190-4. doi: 10.1263/jbb.91.190.

Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control.

Journal of bioscience and bioengineering

P Kahar, T Iwata, J Hiraki, E Y Park, M Okabe

Affiliations

  1. United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

PMID: 16232973 DOI: 10.1263/jbb.91.190

Abstract

The enhancement of epsilon-poly-l-lysine (epsilon-PL) production by Streptomyces albulus strain no. 410 (S410) by means of a pH control strategy was investigated. S140 cells produce epsilon-PL at a high concentration if the culture pH remains at about 4.0; however, if it shifts to higher than 4.0, the accumulated epsilon-PL is depolymerized. We therefore suggest a pH control strategy for cell growth and epsilon-PL production aimed at increasing the amount of epsilon-PL produced. The cultivation was divided into two control phases. In phase I, cell growth was accelerated by maintaining the pH at higher than 5.0; in phase II, epsilon-PL production was increased by maintaining the pH at about 4.0. To avoid an increase in the pH during phase II as a result of glucose depletion, the glucose concentration was kept at around 10 g/l by glucose feeding. This control strategy enhanced the production of epsilon-PL to 48.3 g/l from 5.7 g/l in the case of batch culture.

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