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Eubel H, Braun HP, Millar AH. Blue-native PAGE in plants: a tool in analysis of protein-protein interactions. Plant Methods. 2005;1(1):11doi: 10.1186/1746-4811-1-11.
Eubel, H., Braun, H. P., & Millar, A. H. (2005). Blue-native PAGE in plants: a tool in analysis of protein-protein interactions. Plant methods, 1(1), 11. https://doi.org/10.1186/1746-4811-1-11
Eubel, Holger, et al. "Blue-native PAGE in plants: a tool in analysis of protein-protein interactions." Plant methods vol. 1,1 (2005): 11. doi: https://doi.org/10.1186/1746-4811-1-11
Eubel H, Braun HP, Millar AH. Blue-native PAGE in plants: a tool in analysis of protein-protein interactions. Plant Methods. 2005 Nov 16;1(1):11. doi: 10.1186/1746-4811-1-11. PMID: 16287510; PMCID: PMC1308860.
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Plant Methods. 2005 Nov 16;1(1):11. doi: 10.1186/1746-4811-1-11.

Blue-native PAGE in plants: a tool in analysis of protein-protein interactions.

Plant methods

Holger Eubel, Hans-Peter Braun, A Harvey Millar

Affiliations

  1. ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Hwy, Crawley 6009, Perth, Australia.

PMID: 16287510 PMCID: PMC1308860 DOI: 10.1186/1746-4811-1-11

Abstract

Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.

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