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Beaulieu M, Beaulieu Y, Melinard J, et al. Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate. Appl Environ Microbiol. 1995;61(1):165-9doi: 10.1128/aem.61.1.165-169.1995.
Beaulieu, M., Beaulieu, Y., Melinard, J., Pandian, S., & Goulet, J. (1995). Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate. Applied and environmental microbiology, 61(1), 165-9. https://doi.org/10.1128/aem.61.1.165-169.1995
Beaulieu, M, et al. "Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate." Applied and environmental microbiology vol. 61,1 (1995): 165-9. doi: https://doi.org/10.1128/aem.61.1.165-169.1995
Beaulieu M, Beaulieu Y, Melinard J, Pandian S, Goulet J. Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate. Appl Environ Microbiol. 1995 Jan;61(1):165-9. doi: 10.1128/aem.61.1.165-169.1995. PMID: 16534900; PMCID: PMC1388323.
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Appl Environ Microbiol. 1995 Jan;61(1):165-9. doi: 10.1128/aem.61.1.165-169.1995.

Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate.

Applied and environmental microbiology

M Beaulieu, Y Beaulieu, J Melinard, S Pandian, J Goulet

PMID: 16534900 PMCID: PMC1388323 DOI: 10.1128/aem.61.1.165-169.1995

Abstract

The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammonium sources such as sulfate, nitrate, phosphate, and chloride salts and those of different ammonium sulfate concentrations were evaluated. The best growth and PHB production were obtained with ammonium sulfate; however, NH(inf4)(sup+) concentrations between 0.5 and 1.5 g/liter showed no significant difference. Ammonium sulfate was therefore used as the sole source of NH(inf4)(sup+) for experiments with cane molasses as the growth activator. Optimal growth and PHB production were obtained with 0.3% molasses. However, the yields of biomass (39 to 48%) and PHB (17 to 26%) varied significantly among the different ammonium substrates and cane molasses concentrations.

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