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Zahnley JC, Smith MR. Insoluble Glucan Formation by Leuconostoc mesenteroides B-1355. Appl Environ Microbiol. 1995;61(3):1120-3doi: 10.1128/aem.61.3.1120-1123.1995.
Zahnley, J. C., & Smith, M. R. (1995). Insoluble Glucan Formation by Leuconostoc mesenteroides B-1355. Applied and environmental microbiology, 61(3), 1120-3. https://doi.org/10.1128/aem.61.3.1120-1123.1995
Zahnley, J C, and Smith, M R. "Insoluble Glucan Formation by Leuconostoc mesenteroides B-1355." Applied and environmental microbiology vol. 61,3 (1995): 1120-3. doi: https://doi.org/10.1128/aem.61.3.1120-1123.1995
Zahnley JC, Smith MR. Insoluble Glucan Formation by Leuconostoc mesenteroides B-1355. Appl Environ Microbiol. 1995 Mar;61(3):1120-3. doi: 10.1128/aem.61.3.1120-1123.1995. PMID: 16534961; PMCID: PMC1388393.
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Appl Environ Microbiol. 1995 Mar;61(3):1120-3. doi: 10.1128/aem.61.3.1120-1123.1995.

Insoluble Glucan Formation by Leuconostoc mesenteroides B-1355.

Applied and environmental microbiology

J C Zahnley, M R Smith

PMID: 16534961 PMCID: PMC1388393 DOI: 10.1128/aem.61.3.1120-1123.1995

Abstract

Leuconostoc mesenteroides B-1355 produced at least three glucosyltransferases (GTFs). We previously identified GTF-2 as alternansucrase and GTF-3 as fraction L dextransucrase. We here show that GTF-1 is a previously unreported sucrase that synthesized water-insoluble dextran. Our evidence consisted of the following. (i) GTF-1 was a major component and GTF-2 was a minor component of culture supernatant fractions, but supernatant fractions actively synthesized water-insoluble glucan. (ii) GTF-1 and culture supernatants produced an unusual high-pressure liquid chromatography pattern of malto-oligosaccharides that was not reproduced by GTF-2-GTF-3 mixtures. (iii) GTF-2, GTF-3, and GTF-2-GTF-3 mixtures did not synthesize insoluble glucan from sucrose. Nearly all of the alternansucrase in young (less than 17-h) cultures was associated with the cells.

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