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White GF, Snape JR, Nicklin S. Biodegradation of Glycerol Trinitrate and Pentaerythritol Tetranitrate by Agrobacterium radiobacter. Appl Environ Microbiol. 1996;62(2):637-42doi: 10.1128/aem.62.2.637-642.1996.
White, G. F., Snape, J. R., & Nicklin, S. (1996). Biodegradation of Glycerol Trinitrate and Pentaerythritol Tetranitrate by Agrobacterium radiobacter. Applied and environmental microbiology, 62(2), 637-42. https://doi.org/10.1128/aem.62.2.637-642.1996
White, G F, et al. "Biodegradation of Glycerol Trinitrate and Pentaerythritol Tetranitrate by Agrobacterium radiobacter." Applied and environmental microbiology vol. 62,2 (1996): 637-42. doi: https://doi.org/10.1128/aem.62.2.637-642.1996
White GF, Snape JR, Nicklin S. Biodegradation of Glycerol Trinitrate and Pentaerythritol Tetranitrate by Agrobacterium radiobacter. Appl Environ Microbiol. 1996 Feb;62(2):637-42. doi: 10.1128/aem.62.2.637-642.1996. PMID: 16535244; PMCID: PMC1388782.
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Appl Environ Microbiol. 1996 Feb;62(2):637-42. doi: 10.1128/aem.62.2.637-642.1996.

Biodegradation of Glycerol Trinitrate and Pentaerythritol Tetranitrate by Agrobacterium radiobacter.

Applied and environmental microbiology

G F White, J R Snape, S Nicklin

PMID: 16535244 PMCID: PMC1388782 DOI: 10.1128/aem.62.2.637-642.1996

Abstract

Bacteria capable of metabolizing highly explosive and vasodilatory glycerol trinitrate (GTN) were isolated under aerobic and nitrogen-limiting conditions from soil, river water, and activated sewage sludge. One of these strains (from sewage sludge) chosen for further study was identified as Agrobacterium radiobacter subgroup B. A combination of high-pressure liquid chromatography and nuclear magnetic resonance analyses of the culture medium during the growth of A. radiobacter on basal salts-glycerol-GTN medium showed the sequential conversion of GTN to glycerol dinitrates and glycerol mononitrates. Isomeric glycerol 1,2-dinitrate and glycerol 1,3-dinitrate were produced simultaneously and concomitantly with the disappearance of GTN, with significant regioselectivity for the production of the 1,3-dinitrate. Dinitrates were further degraded to glycerol 1- and 2-mononitrates, but mononitrates were not biodegraded. Cells were also capable of metabolizing pentaerythritol tetranitrate, probably to its trinitrate and dinitrate analogs. Extracts of broth-grown cells contained an enzyme which in the presence of added NADH converted GTN stoichiometrically to nitrite and the mixture of glycerol dinitrates. The specific activity of this enzyme was increased 160-fold by growth on GTN as the sole source of nitrogen.

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