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D'Andrea G, Lizzi AR, Venditti S, et al. Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting. Proteome Sci. 2006;4:4doi: 10.1186/1477-5956-4-4.
D'Andrea, G., Lizzi, A. R., Venditti, S., Di Francesco, L., Giorgi, A., Mignogna, G., Oratore, A., & Bozzi, A. (2006). Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting. Proteome science, 44. https://doi.org/10.1186/1477-5956-4-4
D'Andrea, Gabriele, et al. "Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting." Proteome science vol. 4 (2006): 4. doi: https://doi.org/10.1186/1477-5956-4-4
D'Andrea G, Lizzi AR, Venditti S, Di Francesco L, Giorgi A, Mignogna G, Oratore A, Bozzi A. Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting. Proteome Sci. 2006 Mar 29;4:4. doi: 10.1186/1477-5956-4-4. PMID: 16571109; PMCID: PMC1435870.
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Proteome Sci. 2006 Mar 29;4:4. doi: 10.1186/1477-5956-4-4.

Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting.

Proteome science

Gabriele D'Andrea, Anna R Lizzi, Sara Venditti, Laura Di Francesco, Alessandra Giorgi, Giuseppina Mignogna, Arduino Oratore, Argante Bozzi

Affiliations

  1. Department of Biochemical Sciences and Technologies, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy. [email protected]

PMID: 16571109 PMCID: PMC1435870 DOI: 10.1186/1477-5956-4-4

Abstract

In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.

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