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Stonier T, Yang HM. Studies on Auxin Protectors: XI. Inhibition of Peroxidase-Catalyzed Oxidation of Glutathione by Auxin Protectors and o-Dihydroxyphenols. Plant Physiol. 1973;51(2):391-5doi: 10.1104/pp.51.2.391.
Stonier, T., & Yang, H. M. (1973). Studies on Auxin Protectors: XI. Inhibition of Peroxidase-Catalyzed Oxidation of Glutathione by Auxin Protectors and o-Dihydroxyphenols. Plant physiology, 51(2), 391-5. https://doi.org/10.1104/pp.51.2.391
Stonier, T, and Yang, H M. "Studies on Auxin Protectors: XI. Inhibition of Peroxidase-Catalyzed Oxidation of Glutathione by Auxin Protectors and o-Dihydroxyphenols." Plant physiology vol. 51,2 (1973): 391-5. doi: https://doi.org/10.1104/pp.51.2.391
Stonier T, Yang HM. Studies on Auxin Protectors: XI. Inhibition of Peroxidase-Catalyzed Oxidation of Glutathione by Auxin Protectors and o-Dihydroxyphenols. Plant Physiol. 1973 Feb;51(2):391-5. doi: 10.1104/pp.51.2.391. PMID: 16658336; PMCID: PMC366271.
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Plant Physiol. 1973 Feb;51(2):391-5. doi: 10.1104/pp.51.2.391.

Studies on Auxin Protectors: XI. Inhibition of Peroxidase-Catalyzed Oxidation of Glutathione by Auxin Protectors and o-Dihydroxyphenols.

Plant physiology

T Stonier, H M Yang

Affiliations

  1. Laboratory of Plant Morphogenesis, Manhattan College, Bronx, New York 10471.

PMID: 16658336 PMCID: PMC366271 DOI: 10.1104/pp.51.2.391

Abstract

Commercial horseradish peroxidase, when supplemented with dichlorophenol and either manganese or hydrogen peroxide, will rapidly oxidize glutathione. This peroxidase-catalyzed oxidation of glutathione is completely inhibited by the presence of auxin protectors. Three auxin protectors and three o-dihydroxyphenols were tested; all inhibited the oxidation. Glutathione oxidation by horseradish peroxidase in the presence of dichlorophenol and Mn is also completely inhibited by catalase, implying that the presence of Mn allows the horseradish peroxidase to reduce oxygen to H(2)O(2), then to use the H(2)O(2) as an electron acceptor in the oxidation of glutathione. Catalase, added 2 minutes after the glutathione oxidation had begun, completely inhibited further oxidation but did not restore any gluthathione oxidation intermediates. In contrast, the addition of auxin protectors, or o-dihydroxyphenols, not only inhibited further oxidation of gluthathione by horseradish peroxidase (+ dichlorophenol + Mn), but also caused a reappearance of glutathione as if these antioxidants reduced a glutathione oxidation intermediate. However, when gluthathione was oxidized by horseradish peroxidase in the presence of dichlorophenol and H(2)O(2) (rather than Mn), then the inhibition of further oxidation by auxin protectors or o-dihydroxyphenols was preceded by a brief period of greatly accelerated oxidation. The data provide further evidence that auxin protectors are cellular redox regulators. It is proposed that the monophenol-diphenol-peroxidase system is intimately associated with the metabolic switches that determine whether a cell divides or differentiates.

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