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Henry EW, Valdovinos JG, Jensen TE. Peroxidases in Tobacco Abscission Zone Tissue: II. Time Course Studies of Peroxidase Activity during Ethylene-induced Abscission. Plant Physiol. 1974;54(2):192-6doi: 10.1104/pp.54.2.192.
Henry, E. W., Valdovinos, J. G., & Jensen, T. E. (1974). Peroxidases in Tobacco Abscission Zone Tissue: II. Time Course Studies of Peroxidase Activity during Ethylene-induced Abscission. Plant physiology, 54(2), 192-6. https://doi.org/10.1104/pp.54.2.192
Henry, E W, et al. "Peroxidases in Tobacco Abscission Zone Tissue: II. Time Course Studies of Peroxidase Activity during Ethylene-induced Abscission." Plant physiology vol. 54,2 (1974): 192-6. doi: https://doi.org/10.1104/pp.54.2.192
Henry EW, Valdovinos JG, Jensen TE. Peroxidases in Tobacco Abscission Zone Tissue: II. Time Course Studies of Peroxidase Activity during Ethylene-induced Abscission. Plant Physiol. 1974 Aug;54(2):192-6. doi: 10.1104/pp.54.2.192. PMID: 16658858; PMCID: PMC541529.
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Plant Physiol. 1974 Aug;54(2):192-6. doi: 10.1104/pp.54.2.192.

Peroxidases in Tobacco Abscission Zone Tissue: II. Time Course Studies of Peroxidase Activity during Ethylene-induced Abscission.

Plant physiology

E W Henry, J G Valdovinos, T E Jensen

Affiliations

  1. Department of Biological Sciences, Herbert H. Lehman College of the City University of New York, Bronx, New York 10468.

PMID: 16658858 PMCID: PMC541529 DOI: 10.1104/pp.54.2.192

Abstract

Ethylene-induced abscission in flower pedicels of Nicotiana tabacum L. cv. Little Turkish causes a progressive increase in peroxidase activity during the first 4 hours of a 5-hour time course ethylene treatment period, with decrease in peroxidase activity occurring between 4 hours and 5 hours, when the supernatant extracts of abscission zone segments are tested spectrophotometrically for peroxidase activity, using guaiacol and hydrogen peroxide. Nonethylene-treated tissue has a much lower level of peroxidase activity over the same time course period. In ethylene-treated tissue the decline in break-strength correlates with the beginning of increase in peroxidase activity (3 hours). When the abscission zone area of the pedicel is further divided into proximal, abscission zone, and distal portions, respectively, the ethylene-treated tissue has the highest peroxidase activity in the abscission zone portion, with the maximum peak occurring at 4 hours and decreasing between 4 hours and 5 hours. Acrylamide gel electrophoresis of enzyme breis from ethylene-treated aand nonethylene-treated plants reveals that no new peroxidase isozymes are formed in response to ethylene, indicating an increase in the amount of one or in both of the two already existing isozyme banding patterns. The measurement of protein in the proximal, abscission zone, and distal segments, over a 5-hour ethylene treatment period, indicates that it is being translocated in a distal to proximal direction in the abscission zone pedicel. The possible participatory role for peroxidase in ethylene-induced tobacco flower pedicel abscission are discussed.

References

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