Display options
Cite
Armstrong DJ, Murai N, Taller BJ, et al. Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations. Plant Physiol. 1976;57(1):15-22doi: 10.1104/pp.57.1.15.
Armstrong, D. J., Murai, N., Taller, B. J., & Skoog, F. (1976). Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations. Plant physiology, 57(1), 15-22. https://doi.org/10.1104/pp.57.1.15
Armstrong, D J, et al. "Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations." Plant physiology vol. 57,1 (1976): 15-22. doi: https://doi.org/10.1104/pp.57.1.15
Armstrong DJ, Murai N, Taller BJ, Skoog F. Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations. Plant Physiol. 1976 Jan;57(1):15-22. doi: 10.1104/pp.57.1.15. PMID: 16659417; PMCID: PMC541955.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1976 Jan;57(1):15-22. doi: 10.1104/pp.57.1.15.

Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations.

Plant physiology

D J Armstrong, N Murai, B J Taller, F Skoog

Affiliations

  1. Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706.

PMID: 16659417 PMCID: PMC541955 DOI: 10.1104/pp.57.1.15

Abstract

The incorporation of the cytokinin N(6)-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either N(6)-benzyladenine-8-(14)C or N(6)-benzyladenine-8-(14)C: benzene-(3)H(G) has been examined. N(6)-benzyladenine was incorporated into both the tRNA and rRNA preparations as the intact base. Over 90% of the radioactive N(6)-benzyladenosine recovered from the RNA preparations was associated with the rRNA. Purification of the crude rRNA by either MAK chromatography or Sephadex G-200 gel filtration had no effect on the N(6)-benzyladenosine content of the RNA preparation. The distribution of N(6)-benzyladenosine moieties in tobacco callus tRNA fractionated by BD-cellulose chromatography did not correspond to the distribution of ribosylzeatin activity. N(6)-benzyladenosine was released from the rRNA preparation by treatment with venom phosphodiesterase and phosphatase, ribonuclease T(2) and phosphatase, or ribonuclease T(2) and a 3'-nucleotidase. N(6)-benzyladenosine was not released from the RNA preparation by treatment with either ribonuclease T(2) or phosphatase alone or by successive treatment with ribonuclease T(2) and a 5'-nucleotidase. Brief treatment of the rRNA preparation with ribonuclease T(1) and pancreatic ribonuclease converted the N(6)-benzyladenosine moieties into an ethyl alcohol soluble form. On the basis of these and earlier results, the N(6)-benzyladenosine recovered from the tobacco callus RNA preparations appears to be present as a constituent of RNA and not as a nonpolynucleotide contaminant.

References

  1. J Biol Chem. 1972 Sep 25;247(18):5675-80 - PubMed
  2. Science. 1971 Jul 23;173(3994):293-9 - PubMed
  3. Plant Physiol. 1973 May;51(5):894-7 - PubMed
  4. Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 - PubMed
  5. Plant Physiol. 1975 May;55(5):853-8 - PubMed
  6. Plant Physiol. 1974 Nov;54(5):737-43 - PubMed
  7. Biochem Biophys Res Commun. 1969 Oct 22;37(3):451-6 - PubMed
  8. Plant Physiol. 1965 May;40(3):469-76 - PubMed
  9. Biochemistry. 1969 Nov;8(11):4361-71 - PubMed
  10. Proc Natl Acad Sci U S A. 1969 Jun;63(2):504-11 - PubMed
  11. Biochem Biophys Res Commun. 1971 Oct 1;45(1):1-5 - PubMed
  12. Angew Chem Int Ed Engl. 1969 Oct;8(10):711-27 - PubMed
  13. Biochemistry. 1964 Jun;3:769-73 - PubMed
  14. Plant Physiol. 1966 Jan;41(1):75-82 - PubMed
  15. Biochem J. 1967 Jan;102(1):251-7 - PubMed
  16. Biochemistry. 1971 Jun 8;10(12):2189-94 - PubMed
  17. Biochimie. 1972;54(1):31-9 - PubMed
  18. J Biol Chem. 1967 Oct 10;242(19):4490-4 - PubMed
  19. Biochemistry. 1967 Oct;6(10):3043-56 - PubMed
  20. Prog Nucleic Acid Res Mol Biol. 1970;10:57-86 - PubMed
  21. Anal Biochem. 1960 Jun;1:66-77 - PubMed
  22. FEBS Lett. 1974 Sep 15;46(1):45-50 - PubMed

Publication Types